流式细胞术测定多发性骨髓瘤细胞表面标记的研究

目的通过CD38/SSC设门方法,用流式细胞术(FCM)检测多发性骨髓瘤(MM)细胞表面的相关抗原分子,探讨改善MM检测的方法,为临床提供更加正确的早期诊断、指导治疗及判断预后的依据。方法MM患者37例,另取20例浆细胞反应性增多症(RP)患者为对照组,检测骨髓瘤细胞表面的免疫表型。结果所有标本经流式细胞术CD38/SSC设门后可见到一群CD38阳性细胞,比例为0.5%~80.0%;多数MM细胞表型为CD38 CD19-CD56 (89.2%),且CD56的表达水平与疾病恶性程度密切相关;骨髓瘤细胞CD40、CD138明显比对照组的正常浆细胞高表达;CD45与CD49 e结合,根据表型不同可判断细胞的成熟度。结论利用CD38/SSC设门方法的流式细胞术对MM进行免疫分型,根据细胞表面抗原的异质性,可鉴别良、恶性浆细胞,为判断预后及靶向治疗提供依据。

Study on determining surface antigens in multiple myeloma cells with flow cytometry

Objective To determine surface antigen of multiple myeloma(MM)cells by gating method of CD38/SSC,explore a better method to determine MM,provide a correct evidence that can imprtve earlier diagnosis,therapy and prognosis.Methods Thirty seven cases of confirmed MM,and 20 reactive plasmacytosis(RP) patients as control group were enrolled in the study.To determine surface antigens of multiple myeloma cells with FCM.Results With CD38/SSC gating method,all the samples in their scatterplot can be seen a cluster of CD38 positive cells,the proportion ranged from 0.5% to 80%.Most phenotype of MM was CD38 CD19-CD56 (89.2%),the expression level of CD56 correlated closely with the malignant degree of disease.The expression of CD40 and CD138 in myeloma cells was obviously higher than that in plasma cells.Different phenotype combining with the expression of CD45 and CD49e could distinguish the cells' degree of maturity.Conclusion Using FCM to immunophenotype MM with CD38/SSC gating method and basing on the heterogeneity of cell surface antigens,we can discriminate myeloma cells from nomal plasma cells,provide evidence for targeted therapy and prognosis.