CD3AK细胞的诱导及其体外抗肿瘤活性的实验研究

本研究采用抗ＣＤ３单克隆抗体辅以少量的基因重组人白细胞介素２和植物血凝素诱导外周血淋巴细胞，成功地研制出具有抗瘤活性的ＣＤ３ＭｃＡｂ激活的杀伤细胞。

Experimental Study on CD 3AK Cell Induction and Anti-tumor Activity in Vitro.

With trace CD 3McAb plus rIL-2 and PHA,perpheral blood lymphocyte was induced and the anti-CD 3 monoclonal antibody activated killer cells(CD 3AK)were prepared.The results showed that CD 3AK were stimulated by trace CD 3McAb plus rIL-2 and PHA,and were proliferated on a large scale.CD 3McAb can activated CD 3AK at one stroke in the concentration of 30ng/ml.When CD 3McAb concentration was 300ng/ml high,the proliferation of CD 3AK cells was far more than that of LAK cells. CD 3AK cells largely blong to CD 3 +,CD + 4and CD 8 + cells.The percentage of CD 3 +and CD 8 + cells increased with the culture time elongation,CD 3AK have high killing activity after culture for 4-16 days.The period of highest killing activity was in culture for 7-10 days,and its anti-tumor activity in vitro was significently higher than that of LAK cells (P<0 05).The results indicated that CD 3AK cell is another more efficient killer cell after LAK and TIL cell.