| 一点红组织培养获得再生植株的研究 |
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| 引用本文： | 刘园,吴庆华,覃文流,凌征柱.一点红组织培养获得再生植株的研究[J].广西医学,2006,28(6):798-799. |
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| 作者姓名： | 刘园 吴庆华 覃文流 凌征柱 |
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| 作者单位： | 1. 广西大学,南宁,530005
2. 广西药用植物园、广西药用植物研究所、中国医学科学院药用植物研究所广西分所,南宁,530023 |
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| 基金项目： | 广西壮族自治区科技厅科技攻关项目（桂科攻0322023-3） |
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| 摘 要： | 目的 对一点红进行组织培养研究，探讨在短时间内快速繁殖一点红种苗的方法。方法 以一点红带有腋芽的茎段为外植体，用MS＋BA1．5mg／L＋NAA0．1mg／L培养基。结果 经30d诱导培养，可得丛生芽4～5个；小芽苗转入1／2MS＋NAA1mg/L培养基上诱导生根，20d后可长成具有3～4条根系的再生植株。结论 此途径为人工栽培一点红提供优质种苗。
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| 关 键 词： | 一点红 腋芽 组织培养 再生植株 |
| 收稿时间： | 2006-04-20 |
| 修稿时间： | 2006-05-28 |
Studies on tissue culture and plant regeneration of Emilia sonchifolia (L.) DC. |
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| LIU Yuan,WU Qing-hua,QIN Wen-liu,LING Zheng-zhu.Studies on tissue culture and plant regeneration of Emilia sonchifolia (L.) DC.[J].Guangxi Medical Journal,2006,28(6):798-799. |
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| Authors: | LIU Yuan WU Qing-hua QIN Wen-liu LING Zheng-zhu |
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| Institution: | 1. Guangxi University ( Nanning 530005;Nanning 530023 |
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| Abstract: | Objective To explore if the quick propagation of Emilia sonchifolia (L.) DC. in vitro culture could be used to provide tube-cultured seedlings in short time. Methods Axial buds were used as explants and cultivated on Murashige and Skoog basal medium by adding different portions of hormones. Results After 30-day cultured., axial buds which were cultivated in MS + BA1. 5mg· L + NAA0.1 mg· L-1 could induce fascicular buds, and each node derived 4 to 5 fascicular buds. The induced fascicular buds were transferred to the medium of 1/2 MS + NAA1 mg· L- 1 to induce roots. After the test-tube plantlet transplanted, there was no yellowing phenomenon (caused by yellow virus).Conclusion This research on tissue culture of Emilia sonchifolia (L.)DC. could supply tube-cultured seedlings for large-scale planting. |
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| Keywords: | Emilia sonchifolia(L )DC Axial bud Tissue culture Regenerated plant |
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