肌球蛋白轻链激酶和肌球蛋白轻链9基因在非小细胞肺癌中的表达及意义

目的研究肌球蛋白轻链激酶（MYLK）及肌球蛋白轻链9（MYL9）在非小细胞肺癌（NSCLC）的表达情况及其与NSCLC临床特征的关系。方法采用荧光定量PCR方法检测60例NSCLC组织、癌旁组织和60例正常肺组织中MYLK mRNA和MYL9 mRNA的表达情况,并分析癌组织MYLK mRNA及MYL9 mRNA表达值与临床分期的相关性。结果 MYLK mRNA、MYL9 mRNA在癌组织中的表达量明显低于癌旁组织及正常肺组织（P均〈0.05）,癌旁组织和正常肺组织中MYLK mRNA、MYL9 mRNA表达量无统计学差异（P均〉0.05）。在癌症患者中,淋巴结转移组MYLK mRNA及MYL9 mRNA的相对表达量比无淋巴结转移组高（P〈0.05）。腺癌组织与鳞癌组织MYLK mRNA及MYL9 mRNA的相对表达量无统计学差异（P〈0.05）。MYLK mRNA与MYL9 mRNA的表达呈正相关（P〈0.001）,MYLK mRNA及MYL9 mRNA表达与肿瘤临床分期呈正相关（P〈0.05）。结论 NSCLC组织中MYLK mRNA和MYL9 mRNA表达均明显低于癌旁组织及正常组织,MYLK mRNA及MYL9 mRNA的表达与临床分期呈正相关。

Expression and Significance of Myosin Light Chain Kinase and Myosin Regulatory Light Chain 9 Gene in Non-Small Cell Lung Cancer

Objective To investigate the expressions of myosin light chain kinase（MYLK） and myosin regulatory light chain 9（MYL9） in non-small cell lung cancer（NSCLC） and their correlation with the clinical characteristics of NSCLC. Methods The expressions of MYLK mRNA and MYL9 mRNA were detected by quantitative real-time PCR（qRT-PCR） in 60 cases of NSCLS tissues,60 cases of the corresponding paraneoplastic tissues and 60 cases of normal lung tissues. The correlation between mRNA expressions of MYLK and MYL9 in cancer tissues and clinical TNM stage was analyzed. Results The MYLK mRNA,MYL9 mRNA expressions in cancer tissues were significantly lower than those in paraneoplastic tissues and normal lung tissues（all P〈0. 05）,no statistically significant difference was found in the MYLK mRNA and MYL9 mRNA expressions between paraneoplastic tissues and normal tissues（all P〉0. 05）.Among NSCLC patients,the relative expressions of MYLK mRNA and MYL9 mRNA in lymph node metastasis group were higher than those in non-lymph node metastasis group（P〈0. 05）. There was no statistically significant difference in the relative expressions of MYLK mRNA and MYL9 mRNA between adenocarcinoma tissues and squamous cell carcinoma tissues（P〉0. 05）. The MYLK mRNA expression positively correlated with the MYL9 mRNA expression（P〈0. 001）. The expressions of MYLK mRNA and MYL9 mRNA positively correlated with clinical TNM stage（P〈0. 05）. Conclusion The expressions of MYLK mRNA and MYL9 mRNA in NSCLC tissues are significantly lower than those in paraneoplastic tissues and normal lung tissues,and correlate with clinical TNM stage.