| 组蛋白去乙酰化酶抑制剂LBH589抑制基底样乳腺癌生长和转移 |
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| 引用本文： | 庄慧萍. 组蛋白去乙酰化酶抑制剂LBH589抑制基底样乳腺癌生长和转移[J]. 天津医科大学学报, 2022, 0(5): 478-482 |
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| 作者姓名： | 庄慧萍 |
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| 作者单位： | （天津医科大学肿瘤医院肿瘤研究所生物化学与分子生物学研究室；国家肿瘤临床医学研究中心；天津市“肿瘤防治”重点实验室；天津市恶性肿瘤临床医学研究中心；乳腺癌防治教育部重点实验室，天津300060） |
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| 摘 要： | 目的：探究组蛋白去乙酰化酶抑制剂LBH589对基底样乳腺癌（BLBC）生长和转移的抑制作用。方法：通过MTT法和平板克隆实验检测LBH589对人乳腺癌细胞系MDA-MB-231和小鼠乳腺癌细胞系4T1增殖能力的影响；Transwell实验检测LBH589对细胞迁移和侵袭能力的影响；实时荧光定量PCR和免疫印迹实验检测LBH589对细胞上皮-间质转化的作用；构建4T1乳腺癌细胞Balb/c小鼠脂肪垫成瘤模型，腹腔注射LBH589，观察4T1细胞成瘤和转移能力。结果：体外实验结果显示，与对照组相比，LBH589显著抑制MDA-MB-231和4T1细胞的增殖能力（t=11.37,P<0.05;t=18.18,P<0.05）、克隆形成能力（t=7.76,P<0.05;t=15.22,P<0.05）、迁移（t=8.8,P<0.05;t=18.0,P<0.05）和侵袭能力（t=8.24,P<0.05;t=15.99,P<0.05）；与对照组相比，LBH589上调细胞的上皮标志物CDH1的mRNA(t=7.33,P<0.05)表达水平，抑制细胞的...
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| 关 键 词： | HDACIs LBH589 基底样乳腺癌 增殖 转移 |
Histone deacetylase inhibitor LBH589 inhibits the growth and metastasis of basal-like breast cancer |
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| ZHUANG Hui-ping. Histone deacetylase inhibitor LBH589 inhibits the growth and metastasis of basal-like breast cancer[J]. Journal of Tianjin Medical University, 2022, 0(5): 478-482 |
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| Authors: | ZHUANG Hui-ping |
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| Affiliation: | （Department of Biochemistry and Molecular Biology，Tianjin Medical University Cancer Institute and Hospital；National Clinical Research Center for Cancer; Key Laboratory of Cancer Prevention and Therapy，Tianjin；Tianjin′s Clinical Research Center for Cancer; Key Laboratory of Breast Cancer Prevention and Therapy，Tianjin Medical University，Ministry of Education，Tianjin 300060，China） |
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| Abstract: | Objective: To investigate the inhibitory effect of histone deacetylase inhibitors LBH589 on the growth and metastasis of basal-like breast cancer. Methods: The effects of LBH589 on the proliferation，migration and invasion of human breast cancer cell line MDA-MB-231 and mouse breast cancer cell line 4T1 were determined by MTT assay，colony formation assay and Transwell assay.The effect of LBH589 on epithelial-mesenchymal transformation was detected by RT-qPCR and Western blotting. The abdominal mammary fat pads tumor-forming model of 4T1 breast cancer cells was established in Balb/c mice，and LBH589 was intraperitoneally injected to observe the effect on the tumor-forming ability of 4T1 cells and visceral metastasis. Results: Compared with control group，in vitro results showed that LBH589 significantly inhibited the proliferation（t=11.37，P<0.05；t=18.18，P<0.05），colony formation ability（t=7.76，P<0.05；t=15.22，P<0.05），migration（t= 8.8，P<0.05；t=18.0，P<0.05），and invasive ability（t=8.24，P<0.05；t=15.99，P<0.05）.Compared with control group，LBH589 upregulated the expression level of epithelial marker CDH1 mRNA（t=7.33，P< 0.05），and inhibited mesenchymal marker VIM，FN1 mRNA（t=5.57，P< 0.05；t =6.3，P<0.05） and protein（t=8.37，P<0.05；t=11.3，P<0.05）.In vivo results showed that treatment with LBH589 significantly inhibited tumor growth and lung metastasis. Conclusion:LBH589 can inhibit the proliferation and metastasis of basal-like breast cancer，which has potential clinical application value. |
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| Keywords: | HDACIs LBH589 basal-like breast cancer proliferation metastasis |
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