| 大鼠脑RC3基因的cDNA克隆及序列分析 |
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| 引用本文： | 郭善一,梁东春,油红捷,刘立明,李蓝戈.大鼠脑RC3基因的cDNA克隆及序列分析[J].天津医科大学学报,2002,8(1):45-47. |
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| 作者姓名： | 郭善一 梁东春 油红捷 刘立明 李蓝戈 |
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| 作者单位： | 天津市内分泌研究所,天津,300070 |
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| 摘 要： | 目的：为研究脑特异性基因-RC3的功能，克隆该基因的cDNA。方法：以大鼠脑为材料提取总RNA并分离纯化poly(A)^ RNA，用作模板以嵌套式逆转录PCR方法克隆RC3cDNA，并将其重组入pUC18质粒，然后用双脱氧末端终止法进行碱基序列分析。结果：大鼠脑RC3cDNA序列有一个碱基差别，但不涉及氨基酸的改变。结论：为进一步研究RC3的功能提供了实验基础。
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| 关 键 词： | RC3 发育 嵌套式PCR cDNA克隆 |
| 文章编号： | 1006-8147(2002)01-0045-03 |
| 修稿时间： | 2001年8月30日 |
The cDNA Cloning and Sequencing of Rat Brain RC3 |
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| GUO Shan-yi,LiANG Dong-chun,YOY Hong-jie,LIU Li-ming,LI Lan-ge.The cDNA Cloning and Sequencing of Rat Brain RC3[J].Journal of Tianjin Medical University,2002,8(1):45-47. |
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| Authors: | GUO Shan-yi LiANG Dong-chun YOY Hong-jie LIU Li-ming LI Lan-ge |
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| Abstract: | Objective: To clone the cDNA or a rat RC3 gene,a brain-specific gene. Methods:RC3 cDNA was amplified in the channel of nested RT-PCR with the poly(A)+RNA isolated from the rat brain,and RC3 cDNA fragment was inserted in to pUC18 vector and sequenced by the dideoxy chain termination method.Results:It shows that only one base is different from those reported by Sato-T,which does not involve in change of amino acid.Conclusion:The rat RC3 cDNA obtained basis for futher researching on RC3 function. |
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| Keywords: | RC3 Brain Development nested PCR cDNA cloning |
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