α-干扰素对脐血来源的树突状细胞分化成熟及功能影响的体外研究

目的：在体外探讨α-干扰素（IFN-α）对人脐血来源的树突状细胞（DC）分化成熟及功能的影响。方法：取lO份脐血分离单个核细胞，常规DC诱导培养条件中加入不同剂量的IFN—α（依剂量0、100、500U／m1分为A、B、C3组）。流式细胞仪分析CD83等细胞表面标志，ELISA检测IL-12等细胞因子的表达，MTT法检测DC对同种异体T淋巴细胞的增殖能力。结亲：在常规DC诱导培养条件中加入IFN—α培养的贴壁单个核细胞，在第5天即可见B、C组细胞表面呈树突状突起．第7天B、C组诱导的细胞其DC特异性表面标志CD83、HLA—DR、CD86较A组明显升高，且随IFN-α剂量增加而增加（P〈O．05）；B、C组分泌的细胞因子IL-12、IFN-γ较A组均增高，而IL-10较A组减少（P〈O．05）；B组和C组DC刺激T淋巴细胞增殖的能力显著高于A组（P〈O．05），又以T与DC比为10：1时最明显。结论：在常规DC诱导培养基础上．再加入IFN-α能进一步促进脐血单个核细胞向具有DC典型形态及更强功能的成熟DC转化。

Influence of interferon-α on the differentiation,maturation and functions of dendritic cells from cord blood in vitro

Objective： To study the influence of interferon-α on differentiation, maturation and im- mune function of human cord blood-derived dendritic cells in vitro. Methods： Mononuclear cells were iso- lated from ten samples of cord blood and each sample was divided into 3 groups （A, B and C）. In conven- tional DC induction condition, different doses （0,100 and 500 U/ml） of IFN-α were added into A,B,C groups, respectively. Cell morphous were observed by microscope ,cell surface markers such as CD83 were analysis by FACS, cytokines such as IL-12 were detected by ELISA, and the capability of DC stimulating allogeneic T cell proliferation was assayed by MTF. Results： After 5 days, typical DC morpholology could be seen in B and C groups. On 7th day, surface marker CD83, HLA-DR and CD86 expressed higher in B and C groups than those in A group （P〈0.05）. IL-12 and IFN-γ secreted by DC in B and C groups were higher than those in A group, while IL-IO lower in B and C groups than those in A group （P〈0.05）. The capability of DC stimulating T cell proliferation in B and C groups was higher than that in A group （P〈0.05）, espe- cially when the ratio of DC to T cell was 1：10. Conclusion： The findings suggest that, in conventional DC induction condition, adding IFN-α could promote cord blood-derived mononuclear cells to convert into mature DC with typical morphous and stronger function.