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| 牛磺酸对小鼠脾细胞DNA损伤的影响 | |
| 引用本文： | 张峥,张秀珍,李珏声,吴一平.牛磺酸对小鼠脾细胞DNA损伤的影响[J].青岛大学医学院学报,1999,35(2). |
| 作者姓名： | 张峥 张秀珍 李珏声 吴一平 |
| 作者单位： | 1. 青岛医学院生物化学教研室,青岛266021 2. 青岛医学院医学营养研究所 3. 青岛市市立医院统计科 |
| 摘 要： | 目的探讨牛磺酸对紫外线所致小鼠脾细胞ＤＮＡ损伤的影响。②方法小鼠饲料中分别添加牛磺酸、维生素Ｃ（ＶｉｔＣ）和维生素Ｅ（ＶｉｔＥ）喂养１１２ｄ后，荧光法（ＦＡＤＵ法）检测小鼠脾细胞ＤＮＡ链的断裂程度，并与对照组比较。③结果紫外线照射前，小鼠脾细胞ＤＮＡ链断裂程度各组间差异无显著性（Ｆ＝０．８７５６，Ｐ＞０．０５）；紫外线照射后，牛磺酸、ＶｉｔＣ，ＶｉｔＥ各组脾细胞ＤＮＡ双链剩余率高于对照组，差异均有显著性（Ｆ＝２０．４５，ｑ＝３．０１～４．０８，Ｐ均＜０．０５）。④结论牛磺酸可减轻紫外线对小鼠脾细胞ＤＮＡ分子的损伤。 |
| 关 键 词： | DNA损伤 紫外线 牛磺酸 脾 小鼠 |
THE EFFECT OF TAURINE ON DNA DAMAGE IN SPLENOCYTES OF MICE |
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| \ Zhang Zheng,Zhang Xiuzhen,Li Juesheng,et al\.THE EFFECT OF TAURINE ON DNA DAMAGE IN SPLENOCYTES OF MICE[J].Acta Academiae Medicinae Qingdao Universitatis,1999,35(2). | |
| Authors: | \ Zhang Zheng Zhang Xiuzhen Li Juesheng \ |
| Institution: | \ Zhang Zheng,Zhang Xiuzhen,Li Juesheng,et al\ Department of Biochemistry,Qingdao Medical College,Qingdao 266021 |
| Abstract: | Objective\ To investigate the effect of taurine on DNA damage in spleen cells of mice resulted from ultraviolent irradiation. \ Methods\ After mice were fed with taurine or Vit C or Vit E respectively for 112 days, DNA strand breaks could be detected by fluorometric assay of DNA unwinding(FADU) and were compared with the control group.\ Results\ No significant difference was found on breakage of DNA strands among groups before ultraviolent irradiation ( F= 0.8756 ,P >0.05). After UV irradition ,there was significantly lower breakage of DNA strands in taurine , Vit C and Vit E groups than that in the control group ( F=20.45,q=3.01-4.08,P <0.05).\ Conclusion\ These findings suggest that taurine can protect DNA strands in mice spleen cells from UV irradition. |
| Keywords: | DNA damage ultraviolet rays taurine spleen mice |
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