| CHO细胞表面与人CD59结合的活性短肽序列的筛选 |
| |
| 引用本文： | 崔林林,高美华,赵鹏.CHO细胞表面与人CD59结合的活性短肽序列的筛选[J].青岛大学医学院学报,2007,43(3):196-198. |
| |
| 作者姓名： | 崔林林 高美华 赵鹏 |
| |
| 作者单位： | 青岛大学医学院免疫学教研室,山东,青岛,266071 |
| |
| 摘 要： | 目的筛选获得高表达人CD59中国仓鼠卵巢细胞（CHO）表面与人CD59特异性结合的短肽序列,为下一步研究与肿瘤逃逸相关的CD59活性位点的短肽药物提供实验依据。方法以离子层析柱纯化的CHO细胞的裂解蛋白纯蛋白为靶分子分别进行5轮亲和筛选,并进行竞争结合实验,双夹心ELISA方法鉴定噬菌体阳性克隆。提取阳性单克隆单链DNA测序,并推导出短肽序列。结果随机挑选的16个单克隆中有10个克隆对CHO细胞纯蛋白有特异性结合力,经测序得到两条高度同源的多肽序列。结论通过噬菌体随机肽库对CHO细胞进行纯蛋白筛选得到了能与人CD59特异性结合的短肽序列。
|
| 关 键 词： | 噬菌体肽库 蛋白质CD59 短肽封条 |
| 文章编号： | 1672-4488（2007）03-0196-03 |
| 修稿时间： | 2006-09-192007-01-11 |
SCREENING FOR SHORT ACTIVE PEPTIDE SEQUENCE WITH BINDING TO HUMAN CD59 OF THE SURFACE OF CHO CELL |
| |
| CUI LIN-LIN,GAO MEI-HUA,ZHAO PENG.SCREENING FOR SHORT ACTIVE PEPTIDE SEQUENCE WITH BINDING TO HUMAN CD59 OF THE SURFACE OF CHO CELL[J].Acta Academiae Medicinae Qingdao Universitatis,2007,43(3):196-198. |
| |
| Authors: | CUI LIN-LIN GAO MEI-HUA ZHAO PENG |
| |
| Institution: | Department of Immunology, Qingdao University Medical College, Qingdao 266071, China |
| |
| Abstract: | Objective To screen the sequence of short active peptide binding to human CD59 of the surface of CHO cell, and provide an experimental basis for designing an tumor-sneaking-through-related active short-peptide medicine of CD59. Methods Taking CHO cells protein as target cells by ion-exchange chromatograph,a five-round affinity screening was done randomly. After a competitive test, positive phage clones were identified by sandwich ELISA and sequenced. Results Ten out of 16 phage clones were identified to have higher combination with CD59. Conclusion The sequence of peptide binding to CD59 is ob tained by screening of CHO cell protein through phage random peptide library. |
| |
| Keywords: | phage peptide library protein CD59 short-peptide clamp |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
