心肌损伤和心外膜涂酸对大鼠中枢阿片肽含量的影响

应用放射免疫法测定心肌损伤大鼠和心外膜涂酸大鼠各脑区及垂体中亮氨酸脑啡肽(LEKIS)和β—内啡肽样免疫活性物质(β—EPIS)的含量。结果表明,心肌损伤组大鼠各脑区中L EKIS和β—EPIS的含量与对照组比较,无显著性差别。心外膜涂酸组大鼠的下丘脑、中脑、海马内的LEKIS含量与对照组比较明显增加(P<0.01);纹状体的LEKIS含量亦增加(P<0.05);丘脑、桥脑、延脑与垂体的LEKIS无显著差别;β—EPIS含量仅在延脑增加(P<0.5),其它脑区无显著差别。

THE EFFECT OF MYOCARDIAL INJURY AND CARDIAC PERICARDIUM ACID SMEARING ON CONTENTS OF CENTRAL OPIOID PEPTIDES IN RATS

By means of radioimmunoassy, we studied the effects of myocardial injury (MI) and cardiac pericardium acid smearing (CPAS) on contents of Leu-enkephalin (LEKIS) and B-endorphin immunoreactive substance (B-EPIS) in several brain areas (hypothalamus, thalamus, mesence-phalon hippocampus, corpus striatum, pons varolii, medulla oblongata, and pituitary). Eighteen SD male rats were used and divided into con-trol, myocardeal injury (MI) and CPAS groups. Each group had six rats. All of the rats were anesthetized with urethane. The results show:There were no significant changes in LEKIS and B-EPIS contents in brain areas in MI group compared with those of the controls. The con-tents of LEKIS were significantly increased (P<0.01) in hypothalamus,mesencephalon, hippocampus in CPAS group compared with those of the controls, the contents of LEKIS also were increased in corpus stria-tum(P<0.05). The contents of B-EPLS were elevated in the medulla oblongata(P<0.05). However, there were no significant changes in other brain areas in CPAS group.