纤维蛋白原是冠心病的独立危险因素之一

目的研究纤维蛋白原（Fbg）等危险因素在冠心病（CHD）中的作用。方法对1017例CHD组和470例对照者组分别进行血浆Fbg浓度及其相关基因（（Hae Ⅲ多态性）测定分析。外周血白细胞抽提脱氧核糖核苷酸（DNA），采用酚／氯仿方法；多聚酶链反应（PCR）加酶切技术检测Fbg（Hae Ⅲ多态性。血浆Fbg浓度（200～400mg／dl）测定采用凝血酶法。血脂测定采用标准酶法。高血压病、糖尿病入选标准：①既往有明确高血压病、糖尿病病史者；②本次入院确诊者。每日吸烟1支以上超过1年定为吸烟者。所有数据采用SPSS11．0统计软件进行统计分析。结果（1）年龄、吸烟、高血压、糖尿病、血清胆固醇、三酰甘油和血浆Fbg水平是CHD危险因素；（2）Pearson相关分析显示年龄、高血压、糖尿病、血清胆固醇、高密度脂蛋白胆固醇、Fbg（Hae Ⅲ多态性和吸烟对血浆Fbg水平有明显影响。结论除已公认的CHD危险因素外，血浆Fbg浓度增高可能也是CHD的独立危险因素之一，并明显受其基因（HaeⅢ多态性和吸烟等诸多因素的影响。

Fibrinogen is the One of Risk Factors of Coronary Heart Disease

Objective To assess the clinical significance of plasma fibrinogen and blood lipid et al in patients with coronary heart disease （CHD）. Methods （Hae Ⅲ polymorphisms and plasma fibrinogen levels were simultaneously measured in 1 017 patients with CHD and 470 control subjects. DNA was extracted using the phenol/chloroform method. Plasma fibrinogen concentration was assayed with thrombin method. Total cholesterol（TC） ,Triglyceride （TG） and High density lipoprotein cholesterol （HDL-C） levels were assayed by standard enzyme method. Recording smoking and CHD history et al. other multifactors were simultaneously analysed by Logistic and Pearson methods. Results （ 1 ） plasma fibrinogen is the one of independent risk factors for CHD besides blood lipids and smoking, et al. （2） plasma fibrinogen is significantly effected by （Hae Ⅲ polymorphisms and smoking. Conclusion High plasma fibrinogen level may be an independent risk factor for CHD and influenced by its gene （Hae Ⅲ polymorphisms and smoking et al.