大蒜素对脑缺血再灌注大鼠氧化应激状态的改善作用

目的观察大蒜素对大鼠局灶性脑缺血再灌注损伤的保护作用,并探讨其发生机制。方法将40只体重为150～250 g的健康雄性Wistar大鼠随机平均分为对照组(10只)、模型组(10只)、低剂量组(10 mg/kg)和高剂量组(20 mg/kg)。以改良的Longa线栓法栓塞大鼠右侧大脑中动脉制备脑缺血模型(MCAO),于缺血2 h再灌注24 h时观察,评价不同剂量大蒜素对脑缺血再灌注大鼠的死亡率、神经功能状态及超氧化物歧化酶(SOD)活性的影响。结果与对照组比较,模型组存活率明显降低,神经功能缺失、SOD活性明显降低(P<0.05)。与模型组比较,大蒜素组的死亡率明显降低,神经功能评分增加且SOD活性升高(P<0.05),并且这种影响与剂量存在对应关系。结论大蒜素对大鼠急性脑缺血再灌注损伤有良好的保护作用,其机制可能与提高抗氧化酶活性,改善氧化应激状态有关。

Improvements of Allicinon Cerebral Ischemia-Reperfusion Oxidative Stress Injury in Rats

Objective To study the improving effects of allicin on cerebral ischemia-reperfusion injury in rats and explore the mechanism . Methods The 40 male Wistar rats were randomly divided into four groups. That is, control group（ 10）, model group（ 10）, low-dose group（ 10 mg/kg） and high dose group（20 mg/kg）. Cerebral ischemia model was established in rats by improved Longa suture method through occluding the middle cerebral artery of the right side （MCAO）. The reperfusion was operated 2 hours later and would last for 24 hours. The protective effects of alliein at different doses were evaluated by investigating mortality rate, neurological score and superoxide dismutase （SOD） activity. Results Compared with control group, the survival rate and neurological function score of model group were reduced, with SOD activity declined sig- nificantly （P 〈 0.05 ）. Compared with model group, the survival rate of alliein group was dramatically in- creased, with neurological function score and SOD activity levels raised（ P 〈 0.05 ） , and there is a correspon- dence between the impact and dose. Conclusion Allicin has a good protective effect on acute cerebral ische- mia-reperfusion injury and its mechanism may be related to increased activity of antioxidant enzymes and the improved state of oxidative stress.