|
|||||
|
|
| 紫杉醇提取工艺的优化试验研究 | |
| 引用本文： | 杨宪,杨水平,张雪,谭劼.紫杉醇提取工艺的优化试验研究[J].第三军医大学学报,2007,29(19):1886-1888. |
| 作者姓名： | 杨宪 杨水平 张雪 谭劼 |
| 作者单位： | 1. 西南大学资源环境学院,重庆,400716;重庆赛诺生物药业股份有限公司,重庆,410041 2. 西南大学资源环境学院,重庆,400716 3. 重庆赛诺生物药业股份有限公司,重庆,410041 |
| 摘 要： | 目的 优化并建立红豆杉树皮中紫杉醇的提取工艺.方法 以使用RP-HPLC测定的紫杉醇含量为指标,采用正交试验法对提取过程中乙醇浓度(A)、乙醇用量(B)、提取次数(C)、提取时间(D)4因素进行优选试验.结果 从红豆杉中提取紫杉醇的最主要影响因素是乙醇浓度及乙醇用量,高浓度、适当多的用量有利于提取;提取时间和次数也极为重要,单因素效果分别以每次提取2 h和共提取2次为最佳.结论 T9(A3B3C2D1)、T8(A3B2C1D3)为优化选出的紫杉醇最佳提取工艺条件. |
| 关 键 词： | 红豆杉 紫杉醇 正交试验 |
| 文章编号： | 1000-5404（2007）19-1886-03 |
| 修稿时间： | 2006-10-13 |
Optimization of taxol extraction from Taxus baccata L. |
|
| YANG Xian,YANG Shui-ping,ZHANG Xue,TAN Jie.Optimization of taxol extraction from Taxus baccata L.[J].Acta Academiae Medicinae Militaris Tertiae,2007,29(19):1886-1888. | |
| Authors: | YANG Xian YANG Shui-ping ZHANG Xue TAN Jie |
| Institution: | 1.College of Resource and Environment, Southwest University, Chongqing 400716 ; 2 .Chongqing Sino Biology Pharmaceutical Co. LTD. , Chongqing 410041, China |
| Abstract: | Objective To optimize and establish the extractive technology of taxol from Taxus baccatal L.. Methods HPLC was applied to determine the content of taxol. The orthogonal test was adopted to examine the effects of the 4 factors consisting of ethanol concentration,ethanol volume,extractive times and extractive time. Results Ethanol concentration and ethanol volume were essential factors influencing the extraction of taxol. High concentration and amount of ethanol facilitated the extraction. Extractive times and extractive time were also important. By single-factorial effect,2 h and 2 times in each extraction of taxol were the best. Conclusion The optimum extractive condition is T9 (A3B3C2D1) and T8 (A3B2C1D3). |
| Keywords: | HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |