| 体外培养大鼠海马神经元糖氧剥夺/复氧轴突损伤模型的建立 |
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| 引用本文： | 殷成,秦新月,吴小慧.体外培养大鼠海马神经元糖氧剥夺/复氧轴突损伤模型的建立[J].第三军医大学学报,2009,31(9). |
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| 作者姓名： | 殷成 秦新月 吴小慧 |
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| 作者单位： | 重庆医科大学附属第一医院神经内科,重庆市神经病学重点实验室,重庆,400016 |
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| 摘 要： | 目的 建立体外培养大鼠海马神经元糖氧剥夺/复氧轴突损伤模型.方法 取体外培养7 d的海马神经元,分为正常对照组和糖氧剥夺/复氧组.后者分别在糖氧剥夺0.5、1.0、1.5 h再复糖复氧.常氧状态下检测各组复氧后1、5、24、48、72 h培养液中的LDH活性,并观察神经元形态和轴突长度的变化.结果 糖氧剥夺/复氧后的神经元折光性降低,胞体肿胀,并且轴突逐渐变短,同时LDH水平随时间延长增加.其中糖氧剥夺0.5 h再复氧组效果最佳,其轴突缩短明显且神经元存活率较高.结论 成功建立了体外培养SD大鼠海马神经元糖氧剥夺/复氧轴突损伤模型.
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| 关 键 词： | 糖氧剥夺/复氧模型 海马神经元 体外培养 轴突 |
Establishment of in vitro oxygen-glucose deprivation/reoxygenation axon injury model in rat hippocampal neurons |
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| YIN Cheng,QIN Xin-yue,WU Xiao-hui.Establishment of in vitro oxygen-glucose deprivation/reoxygenation axon injury model in rat hippocampal neurons[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(9). |
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| Authors: | YIN Cheng QIN Xin-yue WU Xiao-hui |
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| Institution: | YIN Cheng,QIN Xin-yue,WU Xiao-hui (Department of Neurology,Key Laboratory of Neurology of Chongqing,First Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To establish an in vitro oxygen-glucose deprivation/reoxygenation axon injury model in rat hippocampal neurons in order to found a basis for research concerning the injury of oxygen glucose deprivation and axons regeneration. MethodsThe hippocampal neurons isolated from rats 24 h after born and cultured for 7 d were exposed to D-hanks solution with nitrogen gas instead of the original culture medium for 0.5, 1.0 and 1.5 h respectively, and then continue to be cultured in the original culture mediu... |
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| Keywords: | oxygen-glucose deprivation/reoxygenation model hippocampal neuron culture in vitro axon |
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