| Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究 |
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| 引用本文： | 刘川,吴小候,张唯力,唐伟,尹志康,何云锋,杜虎.Livin反义寡核苷酸诱导人肾癌细胞凋亡及其机制的研究[J].第三军医大学学报,2008,30(17):1622-1625. |
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| 作者姓名： | 刘川 吴小候 张唯力 唐伟 尹志康 何云锋 杜虎 |
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| 作者单位： | 1. 重庆医科大学,附属第二医院泌尿外科,重庆400010
2. 重庆医科大学,附属第一医院泌尿外科,重庆,400016 |
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| 摘 要： | 目的 探讨Livin反义寡核苷酸(ASODN)对人肾癌细胞株786.O Livin mRNA及蛋白表达的抑制作用以及对细胞凋亡的影响.方法 设计合成全硫代磷酸化修饰的Livin ASODN,脂质体辅助转染至786-O细胞内.采用RT-PCR检测转染后Livin mRNA的变化,细胞免疫化学、激光扫描共聚焦显微镜观察Livin蛋白在细胞内的分布、转染后表达的变化及细胞形态的变化,流式细胞仪检测细胞凋亡率的变化,激酶法测定Caspase3活性的变化.结果 转染Livin ASODN后,RT-PCR显示786-O细胞表达Livin mRNA减少(P＜0.01),激光扫描共聚焦显微镜下见细胞表达Livin蛋白减少、细胞形态发生变化,流式细胞仪检测细胞凋亡率显著增加(P＜0.01),Caspase3活性明显增加(P＜0.01).结论 Livin ASODN能明显下调786-O细胞Livin基因的表达,诱导细胞凋亡.抑制Caspase3活性是Livin抑制细胞凋亡的途径之一.
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| 关 键 词： | 寡核苷酸类 反义 细胞凋亡 肾肿瘤 |
Antisense oligonucleotide targeting livin induces apoptosis of human renal carcinoma cell 786-O in vitro and its mechanism |
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| LIU Chuan,WU Xiao-hou,ZHANG Wei-li,TANG Wei,YIN Zhi-kang,HE Yun-feng,DU Hu.Antisense oligonucleotide targeting livin induces apoptosis of human renal carcinoma cell 786-O in vitro and its mechanism[J].Acta Academiae Medicinae Militaris Tertiae,2008,30(17):1622-1625. |
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| Authors: | LIU Chuan WU Xiao-hou ZHANG Wei-li TANG Wei YIN Zhi-kang HE Yun-feng DU Hu |
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| Institution: | LIU Chuan1,WU Xiao-hou2,ZHANG Wei-li1,TANG Wei2,YIN Zhi-kang2,HE Yun-feng2,DU Hu2 (1Department of Urinary Surgery,The Second College of Clinical Medicine,Chongqing 400010,2Department of Urology,College of Clinical Medicine,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Objective To investigate the effect of antisense oligonucleotides (ASODN) targeting livin on the inhibition of livin mRNA and protein expression and the apoptosis of human renal carcinoma cell line 786-O cells. Methods Specific phosphorothioate antisense oligodeoxynucleotides targeting livin were synthesized and then transfected into 786-O cells. The expressions of livin mRNA were detected by RT-PCR. Expression and location of livin protein were observed by confocal laser scanning microscope (CLSM). Apoptos... |
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| Keywords: | Livin |
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