| DXB／c近交系小鼠的建立及遗传学检测 |
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| 引用本文： | 魏泓,周建华.DXB／c近交系小鼠的建立及遗传学检测[J].第三军医大学学报,1993,15(5):430-433. |
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| 作者姓名： | 魏泓 周建华 |
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| 作者单位： | 第三军医大学实验动物学教研室 630038
(魏泓，周建华，牛荣，陈丙波)，第三军医大学实验动物学教研室 630038(陈书荣) |
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| 摘 要： | 将ＤＢＡ／２雌鼠与Ｃ５７ＢＬ／６雄鼠杂交后，利用其Ｆ２代中结进行兄妹交配，建立了ＤＸＢ／ｃ近交系小鼠。目前已近交２８代，历时１３年，经皮肤移植试验、下颌骨形态分析、混合淋巴细胞培养、毛色基因及生化标志基因检测，表明ＤＸＢ／ｃ系小鼠的基因已经纯合，符合一个近交系小鼠的标准，毛色基因及生化标志基因测试结果同时表明ＤＸＢ／ｃ系小鼠的遗传背景组合了两亲代品系的遗传基因。
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| 关 键 词： | 小鼠 近交系 遗传学 近交系杂交 |
Establishment and genetic checkup of an inbred strain of DXB/c mouse |
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| Wei Hong,Zhou Jianhua,Niu Rong,Chen Bingbo,Chen Shurong.Establishment and genetic checkup of an inbred strain of DXB/c mouse[J].Acta Academiae Medicinae Militaris Tertiae,1993,15(5):430-433. |
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| Authors: | Wei Hong Zhou Jianhua Niu Rong Chen Bingbo Chen Shurong |
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| Abstract: | An inbred strain of DXB/c mouse has been established by hybridization between DBA/2 female mice and C57BL/6 male ones and subsequently by sibmating their offsprings beginning from the F2 generation.Now DXB/c mouse has been passed for 28 generations of full sibmating since 1979.Genetic checkup by means of skin grafting,mandibular morphology analysis,mixed lymphocyte cultivation,coat colour gene testing,and biochemical marker gene examination confirmed that the full homozygosity of alleles has been achieved in DXB/c mouse and DXB/c mouse comforms to the criteria of an inbred strain of mouse.In addition,the genetic background of DXB/c mouse is composed of the genes of its progenitors DBA/2 and C57BL/6 as shown by coat colour gene testing and biochemical marker gene examination. |
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| Keywords: | mice inbred strains mice inbred DBA mice inbred C_(57)BL genetics |
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