实验性糖尿病肾病大鼠模型建立及优化

目的 比较几种不同因素组合对实验性糖尿病肾病大鼠模型建立中大鼠体质量、血糖及尿微量白蛋白的影响,确定建立糖尿病肾病大鼠模型的最佳方案.方法 雄性SD大鼠30只,随机分为5组,每组6只.A组(正常对照组),B组(高糖高脂饲料),C组高糖高脂饲料 链脲佐菌素(Streptozotocin,STZ)组],D组(高糖高脂饲料 阿霉素 STZ组),E组(高糖高脂饲料 单肾切除 STZ组).大鼠造模后每2周测体质量、进食量、饮水量、血糖及尿微量白蛋白.造模12周后,处死大鼠.结果 E组大鼠明显多食、多饮、消瘦、血糖升高,尿微量白蛋白不仅与正常组比较有显著差异,并且与前几个模型组比较也都有显著性差异.肾/体质量比显著增大.结论 以大鼠高糖高脂饲料4周后单肾切除,2周后小剂量腹腔注射链脲佐菌素的模型为最佳.

Optimal selection of rat models of experimental diabetic nephropathy

Objective To compare different combinations of several factors in establishing rat model of experimental diabetic nephropathy and decide the preferred plan. Methods Thirty SD rats were randomly divided into 5 groups with six rats in every group. A was normal group; B was high-lipids and high-sucrose feeding group; C was high-lipids and high-sucrose feeding streptozotocin (STZ) group; D was high-lipids and high-sucrose feeding doxorubicin hydrochloride STZ group; E was high-lipids and high-sucrose feeding mono-nephrectomy STZ group. The body weight, food-intake, water-intake volume, blood sugar, microprotein in urine were detected in every two weeks. Twelve weeks later after model establishment, the rats were put to death, then heart blood were prepared and kidney was weighed. Results Although taking more food and water, the rats of group E were more emaciated, of higher blood sugar and microprotein in urine, and increased kidney/body weight ratio. There was significant difference between the rats in group E and groups A, B, C, D. Conclusion The rat model established by first high-lipids and high-sucrose feeding for 4 weeks, then mono-nephrectomy, and 2 weeks later intraperitoneal administration of STZ is ideal.