组织工程真皮抗张强度的初步研究

目的 采用高敏度小张力膜状生物材料力学性能测定仪检测组织工程真皮的抗张强度。方法 分别培养含有不同数量成纤维细胞的胶原凝胶和复方壳多糖真皮替代物，15d后检测抗张强度。结果 组织工程真皮抗张强度随成纤维细胞数量增多而增强（P〈0．01），复方壳多糖人工真皮的抗张强度高于胶原凝胶真皮（P〈0.01）。结论 复方壳多糖真皮替代物具有良好的抗张强度，为临床应用提供稳定机械性能。

Primary study of VEGF secreted by cultured tissue engineered dermal equivalents

Objective To measure the tensile strength of tissue engineering dermal equivalent with mechanical property device for high-acuity micro-tension lemma.Methods Collagen dermal equivalent and composite chitosan dermal equivalent with different quantities of fibroblasts were cultured for 15 d and were measured of the tensile strength.Results The tensile strength of tissue engineering dermal equivalents was strengthened with the increase of fibroblasts(P<0.01).The tensile strength of composite chitosan dermal equivalent was stronger than that of collagen dermal equivalent with the same amount of fibroblasts(P<0.01).Conclusion Composite chitosan skin equivalent has better tensile strength amd stable mechanic property for clinic application.