慢性缺氧和二氧化碳潴留大鼠脑阴离子转运蛋白AE3 mRNA表达

通过向密闭舱内灌注混合气体的方法，分别复制慢性缺氧、慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒大鼠模型。以地高辛标记的脑阴离子转运蛋白（ＡＥ３）ｃＤＮＡ探针，用核酸原位杂交及点杂交技术，对ＡＥ３ｍＲＮＡ的分布及变化进行检测，结果显示：①ＡＥ３ｍＲＮＡ广泛分布于脑组织，其中以呼吸调整中枢所在部位脑桥深部灰质最为富集。②慢性缺氧组ＡＥ３ｍＲＮＡ的相对含量显著地低于对照组（Ｐ＜０．０５），慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒组均非常显著地低于对照组（Ｐ＜０．０１）；③慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒组均非常显著地低于慢性缺氧组（Ｐ＜０．０１），但二组之间无显著性差异（Ｐ＞０．０５）。提示ＡＥ３在呼吸衰竭的发生及其导致的肺性脑病中可能具有一定作用。

Expression of anion exchanger mRNA in brains of rats with chronic hypoxia and hypercapnia

After three models of hypoxia, hypoxia with hypercapnia and chronic acidosis were established in rats by pouring the mixed gases into the chamber, the distribution and changes of anion exchanger 3 (AE 3) in the brain of the rats were studied with in situ and dot blot hybridization using digoxigenin labelled cDNA probe. It was found that: (1) AE 3 and mRNA distributed widely in the brain tissue, especially in pontine gray matter where the respiratory center was located. (2) The relative contents of AE 3 and mRNA in the brains of experimental rats were significantly lower than those of the controls. (3) The relative contents of AE 3 and mRNA in the brains of rats with chronic hypoxia and hypercapnia and chronic respiratory acidosis were also significantly lower than those in rats with chronic hypoxia ( P <0.01) but there was no significant difference between chronic hypoxia and hypercapnia and chronic respiratory acidosis. These findings suggest that AE 3 may play an important role in the occurrence of respiratory failure and subsequent pulmonary encephalophathy.