| 腺病毒介导的脑源性神经营养因子对大鼠脑出血后内源性神经干细胞分化的影响 |
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| 引用本文： | 余震,陈莉芬,唐玲,胡长林. 腺病毒介导的脑源性神经营养因子对大鼠脑出血后内源性神经干细胞分化的影响[J]. 第三军医大学学报, 2010, 32(15) |
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| 作者姓名： | 余震 陈莉芬 唐玲 胡长林 |
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| 作者单位： | 重庆医科大学附属第二医院神经内科,重庆,400010;重庆市第二人民医院神经内科,重庆,402160 |
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| 基金项目： | 重庆市卫生局重点课题，重庆医科大学校级创新课题 |
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| 摘 要： | 目的 观察腺病毒介导的脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)对大鼠脑出血后内源性神经干细胞(nerve stem cells, NSCs)分化的影响.方法 建立大鼠自体血脑出血(intracerebral hemorrhage,ICH)模型,将126只大鼠分为生理盐水组(NS组)、单纯腺病毒载体干预组(Ad组)和BDNF干预组(Ad-BDNF组),每组42只.模型制作后分别将NS、Ad、Ad-BDNF注射到出血侧血肿周围.BrdU标记内源性NSCs,于1、3、7、14、2l、28 d进行神经缺失功能评分.免疫荧光单标法观察3组血肿周围GAP-43的表达.免疫荧光双标法观察脑出血大鼠28 d时NSCs的分化.结果 Ad-BDNF组在第7、14、21、28 天神经缺失功能评分优于NS组和Ad组 (P<0.01).GAP-43在脑出血出血同侧3 d 时表达明显增加,7 d 达高峰,以后逐渐减少.Ad-BDNF组在第3、7、14、21、28天的GAP-43表达明显增加,与NS、Ad组比较差异有统计学意义(P<0.05).28 d时Ad-BDNF组BrdU/NeuN双标阳性细胞率为(39.69±17.45)%、BrdU/GFAP双标阳性细胞率为(63.53±21.68)%,与NS组、Ad组比较明显增加(P<0.05).结论 腺病毒介导的BDNF可促进脑出血后内源性NSCs的分化和轴突再生,有利于神经功能的恢复.
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| 关 键 词： | 脑源性神经营养因子 神经干细胞 脑出血 大鼠 |
Effect of adenovirus-mediated brain-derived neurotrophic factor on differentiation of endogenetic nerve stem cells from rats after intracerebral hemorrhage |
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| Yu Zhen,Chen LiFeng,Tang Ling,Hu Changlin. Effect of adenovirus-mediated brain-derived neurotrophic factor on differentiation of endogenetic nerve stem cells from rats after intracerebral hemorrhage[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(15) |
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| Authors: | Yu Zhen Chen LiFeng Tang Ling Hu Changlin |
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| Affiliation: | Yu Zhen1,Chen LiFeng1,Tang Ling2,Hu Changlin1(1Department of Neurology,Second Affiliated Hospital,Chongqing Medical University,Chongqing,400010,2Department of Neurology,Second People's Hospital of Chongqing,402160,China) |
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| Abstract: | Objective To observe the effects of adenovirus-mediated brain-derived neurotrophic factor(Ad-BDNF) on differentiation of endogenetic nerve stem cells(NSCs) after intracerebral hemorrhage(ICH).Methods A rat ICH model was established by right intrastriatal administration of autologous whole blood.One hundred and twenty-six rats were divided into normal saline(NS) group,adenovirus interference group(Ad group) and Ad-BDNF interference group,42 rats in each group.NS,Ad and Ad-BDNF were injected into the perihema... |
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| Keywords: | brain-derived neurotrophic factor nerve stem cells intracerebral hemorrhage rat |
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