| 小鼠肝脏特异的微RNA在HeLa细胞中的转染与表达 |
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| 引用本文： | 孙建国,廖荣霞,陈正堂,周建新.小鼠肝脏特异的微RNA在HeLa细胞中的转染与表达[J].第三军医大学学报,2005,27(3):196-199. |
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| 作者姓名： | 孙建国 廖荣霞 陈正堂 周建新 |
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| 作者单位： | 1. 第三军医大学,新桥医院全军肿瘤治疗中心,重庆,400037
2. 第三军医大学,基础医学部生物化学与分子生物学教研室,重庆,400038 |
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| 摘 要： | 目的观察miR-122在HeLa细胞内表达情况.方法将构建的miR-122真核表达质粒pAVU6 27-mir122以电穿孔法转染HeLa细胞,经G418筛选获得稳定转染的细胞克隆,通过Northern杂交和点杂交验证miR-122在HeLa细胞中的表达.结果阳性转染细胞与小鼠肝脏细胞Northern杂交及斑点杂交均出现放射自显影,而转染空质粒的HeLa细胞则无放射显影.结论 miR-122在HeLa细胞中获得正确表达.
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| 关 键 词： | 微RNA 小干涉RNA 非编码RNA 真核表达 |
| 文章编号： | 1000-5404(2005)03-0196-04 |
| 修稿时间： | 2004年9月16日 |
Gene transfection and expression of murine liver-specific microRNA in HeLa cell line |
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| SUN Jian-guo ,LIAO Rong-xia ,CHEN Zheng-tang ,ZHOU Jian-xin.Gene transfection and expression of murine liver-specific microRNA in HeLa cell line[J].Acta Academiae Medicinae Militaris Tertiae,2005,27(3):196-199. |
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| Authors: | SUN Jian-guo LIAO Rong-xia CHEN Zheng-tang ZHOU Jian-xin |
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| Institution: | SUN Jian-guo 1,LIAO Rong-xia 2,CHEN Zheng-tang 1,ZHOU Jian-xin 2 |
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| Abstract: | Objective To express miR-122 in HeLa cells. Methods pAVU6 27-mir122, an eukaryotic expression vector of miR-122, was transfected into HeLa cell line by electroporation. G418-resistant clones were screened for cell clones with stable transfection. Northern blotting and dot blotting were used to detect miR-122 expression in the transfected HeLa cells. Results Northern blot and dot blot analyses demonstrate miR-122 expression in both transfected HeLa cells and murine hepatocytes, but miR-122 expression was not found in HeLa cells transfected with pAVU6 27. Conclusion The expression of miR-122 in HeLa cells may provide the basis for further studies of its targets and biological functions. |
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| Keywords: | microRNA small interfering RNA non-coding RNA eukaryotic expression |
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