| 基因表达谱芯片筛选强直性脊柱炎差异表达基因的研究 |
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| 引用本文： | 张莹,初同伟.基因表达谱芯片筛选强直性脊柱炎差异表达基因的研究[J].第三军医大学学报,2011,33(10):1044-1047. |
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| 作者姓名： | 张莹 初同伟 |
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| 作者单位： | 第三军医大学新桥医院骨科,重庆,400037 |
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| 摘 要： | 目的应用表达谱芯片比较人强直性脊柱炎(ankylosing spondylitis,AS)组织与正常骶髂关节组织的基因表达差异,筛选AS疾病相关基因。方法选取本室采集的3例活动期AS标本为实验组,1例骨折标本为对照组。分别提取标本骶髂关节的滑膜组织与椎旁组织的RNA,制备探针后用于表达谱芯片杂交,筛选AS组织差异表达基因,并采用半定量RT-PCR法验证芯片结果。结果与正常组织样本相比,在AS组织中筛选获得差异表达基因237条,表达上调的已知基因91条,表达下调的已知基因78条;对其中20条基因进行半定量RT-PCR检测,结果与表达谱芯片结果一致。结论筛选出与AS相关的多个差异表达基因。
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| 关 键 词： | 强直性脊柱炎 基因表达谱芯片 差异表达基因 |
Screening of differentially expressed genes in ankylosing spondylitis by cDNA microarray |
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| Zhang Ying,Chu Tongwei.Screening of differentially expressed genes in ankylosing spondylitis by cDNA microarray[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(10):1044-1047. |
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| Authors: | Zhang Ying Chu Tongwei |
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| Institution: | Zhang Ying,Chu Tongwei(Department of Orthopedics,Xinqiao Hospital,Third Military Medical University,Chongqing,400037,China) |
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| Abstract: | Objective To compare the gene expression difference between human ankylosing spondylitis(AS) and normal sacroiliac joint tissues,and to screen AS-associated genes via cDNA microarray.Methods Three AS samples in active phase were collected and used as a test group,and a fracture sample was used as a control group.Total RNA was isolated from synovial and paravertebral tissues of both AS and normal sacroiliac joints,and used to construct hybridization probes.The probes were hybridized to cDNA microarray GeneCh... |
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| Keywords: | ankylosing spondylitis cDNA microarray differentially expressed genes |
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