细胞因子及胸腺微环境影响Treg细胞增殖机制研究

目的通过体外培养体系添加细胞因子和在体细胞因子胸腺注射的方法,探索扩增低比例低增殖活性的Treg细胞的有效体内外方法.方法采用不同浓度细胞因子IL-10和TGF-β1作为诱导剂,分析二因子体外诱导Treg细胞增殖的有效性及最适浓度,同时以二因子胸腺注射的方法,分析在成年鼠体内诱导Treg细胞增殖的可能性.结果 150 ng/ml IL-10浓度能够明显诱导Treg细胞增殖,经48、96、144 h诱导后,其增殖比例分别达到10%、18%和20%,而TGF-β1的使用浓度需达到300 ng/ml;IL-10胸腺注射后同样可提高Treg细胞的比例第7天(5.50±1.38)%,P<0.05;第10天(6.65±2.54)%,P<0.01];TGF-β1诱导后第5天的比例为(5.19±1.69)%,较对照组也有明显升高(P<0.05).结论体外培养体系添加诱导因子增殖的方法是扩增低比例低增殖活性Treg细胞的首选方法,IL-10体内外诱导Treg细胞增殖的效能均强于TGF-β1.

Effects of different cytokines and micro-circumstance of thymus on regulatory T cell proliferation in vitro and in vivo

Objective To explore the effective methods for enriching regulatory T (Treg) cells through the stimulation of cytokines both in vitro and in vivo . Methods Different concentrations of IL-10 or TGF-B1 were employed as the inducer in the culture system to stimulate the proliferation of Treg cells. Cell count was conducted at 48, 96 and 144 h after induction to look for the effective and optimal concentration of the two cytokines in vitro. The possibility of the two cytokines to induce Treg cell proliferation in vivo was analyzed by means of intrathymic injection of IL-10 or TGF-B1 . Results IL-10 and TGF-B1 could induce Treg cell proliferation in vitro and in vivo significantly. Intrathymic injection of IL-10 at the dose of 150 ng/ml and TGF-B1 at the dose of 300 ng/ml could stimulate Treg cell proliferation effectively. Conclusion Addition of cytokines into the culture system is an appropriate method for Treg proliferation. IL-10 is more effective than TGF-B1 in enriching Treg cells in vitro.