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葡萄糖调节蛋白78在大鼠非酒精性脂肪性肝炎中的表达及意义
引用本文:闵敏,陈东风,王军,艾正琳,樊丽琳,严丽丽.葡萄糖调节蛋白78在大鼠非酒精性脂肪性肝炎中的表达及意义[J].第三军医大学学报,2009,31(1).
作者姓名:闵敏  陈东风  王军  艾正琳  樊丽琳  严丽丽
作者单位:第三军医大学大坪医院野战外科研究所消化内科,重庆,400042
摘    要:目的 观察高脂饮食诱导大鼠非酒精性脂肪性肝炎(nonalcoholic steatohepatitis,NASH)过程中内质网应激标志物葡萄糖调节蛋白78的表达变化,探讨内质网应激在NASH发病机制中的作用.方法 建立大鼠高脂饮食诱导NASH模型,检测血清ALT、AST、FFA、TG和肝组织MDA的变化,用RT-PCR与Western blot方法 检测NASH形成过程中肝组织GRF78在基因和蛋白水平的表达变化.结果 NASH组大鼠血清ALT、AST、FFA、TG和肝组织MDA水平均较对照组有不同程度的升高.肝组织GRP78基因和蛋白表达也随NASH加重而增强,16周时升高最为显著(P<0.01).结论 高脂饮食可诱导GRP78表达增强,启动内质网应激,导致脂质异常沉积,其可能与NAsH的发病机制密切相关.

关 键 词:非酒精性脂肪性肝炎  内质网应激  葡萄糖调节蛋白78

Expression and significance of glucose regulated protein GRP78 in nonalcoholic steatohepatitis of rats
MIN Min,CHEN Dong-feng,WANG Jan,AI Zheng-lin,FAN Li-lin,YAN Li-li.Expression and significance of glucose regulated protein GRP78 in nonalcoholic steatohepatitis of rats[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(1).
Authors:MIN Min  CHEN Dong-feng  WANG Jan  AI Zheng-lin  FAN Li-lin  YAN Li-li
Institution:MIN Min,CHEN Dong-feng,WANG Jun,AI Zheng-lin,FAN Li-lin,YAN Li-li(Department of Gastroenterology,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China)
Abstract:Objective To investigate the expression of endoplasmic reticulum molecular chaperone,glucose regulated protein 78(GRP78),in the liver tissues during fatty diet-induced nonalcoholic steatohepatitis(NASH)in rats and explore the role of endoplasmic reticulum stress in pathogenesis of nonalcoholic steatohepatitis.Methods Rat model of nonalcoholic steatohepatitis were established with fatty dieting.Blood samples were collected to detect serum aspartate transaminase(AST),alanine aminotransferase(ALT),free fatty a...
Keywords:nonalcoholic steatohepatitis  endoplasmic reticulum stress  glucose regulated protein 78  
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