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| 乳腺癌miRNAs表达谱检测及初步分析 | |
| 引用本文： | 廖荣霞,孙建国,张亮,陈敏,陈彬,娄桂予,周度金,陈正堂.乳腺癌miRNAs表达谱检测及初步分析[J].第三军医大学学报,2006,28(22):2209-2212. |
| 作者姓名： | 廖荣霞 孙建国 张亮 陈敏 陈彬 娄桂予 周度金 陈正堂 |
| 作者单位： | 1. 第三军医大学,基础医学部生物化学与分子生物学教研室,重庆,400038 2. 第三军医大学,新桥医院肿瘤科,全军肿瘤诊治中心,重庆市肿瘤生物技术研究所,重庆,400037 3. 北京博奥生物芯片有限责任公司,北京,102206 |
| 基金项目： | 国家自然科学基金，军队科技攻关项目，中国博士后科学基金 |
| 摘 要： | 目的 制作哺乳动物micro RNAs(miRNAs)检测微阵列,筛选乳腺癌miRNAs表达谱并进行初步分析.方法 利用已知的人及小鼠的miRNAs序列,设计了496条探针,制作miRNAs微阵列并验证其可靠性;培养乳腺癌细胞株MCF-7和正常乳腺上皮细胞株MCF-10A,分别抽提细胞总RNA,分离小分子RNA,经T4RNA连接酶荧光标记后与微阵列杂交,通过芯片扫描和数据分析,获得乳腺癌miRNAs表达谱.结果 成功制作哺乳动物miRNAs微阵列,筛选获得57个乳腺癌相关miRNAs,其中,29个为低表达,28个为高表达.结论 建立了哺乳动物miRNAs检测微阵列的技术平台,并筛选到乳腺癌miRNAs表达谱,为进一步研究其在乳腺癌发病机制中的作用打下基础. |
| 关 键 词： | 微阵列 乳腺癌 |
| 文章编号： | 1000-5404（2006）22-2209-04 |
| 收稿时间： | 2006-03-22 |
| 修稿时间： | 2006-03-222006-07-14 |
Microarray-based miRNAs profiling in human breast cancer |
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| LIAO Rong-xia,SUN Jian-guo,ZHANG Liang,CHEN Min,CHEN Bin,LOU Gui-yu,ZHOU Du-jin,CHEN Zheng-tang.Microarray-based miRNAs profiling in human breast cancer[J].Acta Academiae Medicinae Militaris Tertiae,2006,28(22):2209-2212. | |
| Authors: | LIAO Rong-xia SUN Jian-guo ZHANG Liang CHEN Min CHEN Bin LOU Gui-yu ZHOU Du-jin CHEN Zheng-tang |
| Institution: | 1 Department of Biochemistry and Molecular Biology, College of Medicine, Chongqing 400038; 2 Cancer Research Centery Xinqiao Hospital, Third Military Medical University, Chofigqing 400037 ;3 CapitalBio Corporation, Beijing 102206, China |
| Abstract: | Objective To make microRNAs (miRNAs) microarray for mammal miRNAs profiling and to screen breast cancer-specific miRNAs and analyze their functions preliminarily. Methods First, 496 probes designed according to known miRNAs sequences of human and mouse. Then, miRNAs microarray was made and subjected to credibility verification. Next, MCF-7, breast cancer cell line, and MCF-10A, normal human mammary epithelial cell line were cultured for respective total RNA extraction. Small molecular RNA was separated and fluorescence labeling was performed by T_4 RNA ligase. Finally, microarray hybridization was performed to obtain miRNAs signature of human breast cancer. Results We successfully made credible miRNAs microarray and obtained 57 breast cancer-related miRNAs by microarray-based screening, including 29 highly-expressed miRNAs and 28 lowly-expressed ones. Conclusion We built up the technical platform of mammal miRNAs detection and obtained miRNAs signature of human breast cancer so as to lay necessary basis for research on the function of miRNAs in breast cancer. |
| Keywords: | miRNAs |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |