| 硫芥淋巴细胞毒性作用与caspase-3表达 |
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| 引用本文： | 赛燕,刘勇,朱明学,张黎明,董兆君.硫芥淋巴细胞毒性作用与caspase-3表达[J].第三军医大学学报,2003,25(13):1127-1130. |
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| 作者姓名： | 赛燕 刘勇 朱明学 张黎明 董兆君 |
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| 作者单位： | 1. 第三军医大学预防医学系军事毒理学教研室,重庆,400038
2. 第二军医大学海医系,上海,200433 |
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| 基金项目： | 全军“十五”重点课题 ( 0 1L0 54 ) |
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| 摘 要： | 目的 探讨硫芥对淋巴细胞的毒性作用方式和分子机制。方法 利用硫芥诱导的体外培养淋巴细胞，采用荧光分光光度法检测DNA的损伤，运用逆转录．聚合酶链式反应(RBPCR)和Western blot方法检测胱氨酸蛋白酶caspase-3的基因和蛋白表达及活化。结果 硫芥可导致淋巴细胞DNA损伤，Caspase-3在基因水平和蛋白水平上表达增加，同时发生活化作用，具有时间依赖效应。结论 硫芥通过caspase依赖途径诱导淋巴细胞毒作用，Caspase-3的表达与活化参与该过程，影响着淋巴细胞的毒性作用。
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| 关 键 词： | 硫芥 淋巴细胞 胱氨酸蛋白酶-3 凋亡 |
| 文章编号： | 1000-5404(2003)13-1127-04 |
| 修稿时间： | 2003年3月12日 |
Cytotoxic action of sulfur mustard on cultured lymphocytes and their caspase-3 expression |
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| SAI Yan,LIU Yong,ZHU Ming xue,ZHANG Li Ming,DONG Zhao jun.Cytotoxic action of sulfur mustard on cultured lymphocytes and their caspase-3 expression[J].Acta Academiae Medicinae Militaris Tertiae,2003,25(13):1127-1130. |
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| Authors: | SAI Yan LIU Yong ZHU Ming xue ZHANG Li Ming DONG Zhao jun |
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| Abstract: | Objective To investigate the manner and molecular mechanism of lymphocyte cytotoxic action induced by sulfur mustard. Methods Lymphocytes were separated from the spleen of Wistar rats and were cultured. DNA strand breaks were detected by fluorospectrophotometry. RT PCR and Western blotting were employed to detect the gene and protein expressions of caspase 3 and its activation. Results Sulfur mustard induced DNA strand breaks in the lymphocytes. Gene and protein expressions of caspase 3 were increased in a time dependent manner. Conclusion Sulfur mustard induces lymphocyte cytotoxic action by means of depending on the expression and activation of caspase 3. |
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| Keywords: | sulfur mustard lymphocyte caspase 3 apoptosis |
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