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缺氧复合梭曼中毒PC12细胞中[Ca2+]、cAMP、CaM和Ca2+/CaM-PK Ⅱ的变化
引用本文:赵吉清,吴强,王仕丽,魏相德,董兆君,李云鹏,刘勇.缺氧复合梭曼中毒PC12细胞中[Ca2+]、cAMP、CaM和Ca2+/CaM-PK Ⅱ的变化[J].第三军医大学学报,2001,23(2):169-171.
作者姓名:赵吉清  吴强  王仕丽  魏相德  董兆君  李云鹏  刘勇
作者单位:第三军医大学预防医学系军事毒理学教研室,
基金项目:军队指令性项目,96L015,
摘    要:目的 观察缺氧复合梭曼中毒的大鼠肾上腺嗜铬细胞瘤PC12细胞内游离钙的浓度[Ca∧2 ]、cAMP、钙调蛋白(CaM)和钙调蛋白Ⅱ(Ca∧2 /CaM-PK Ⅱ)的变化。方法 采用放免技术,从细胞水平进一步观察缺氧复合梭曼中毒对PC12细胞的毒性、细胞内[Ca∧2 ]变化、cAMP、CaM和Ca∧2 /CaM-PK Ⅱ的变化。结果 缺氧中毒组PC12细胞cAMP、CaM含量在中毒后24h明显高于单纯中毒组、缺氧对照组和正常对照组;缺氧中毒组PC12细胞Ca∧2 /CaM-PK Ⅱ活性在中毒后24h明显低于单纯中毒组、缺氧对照组和正常对照组。结论 [Ca∧2 ]、cAMP、CaM和Ca∧2 /CaM-PK Ⅱ在缺氧复合梭曼中毒PC12细胞损伤机制中起了重要的作用。

关 键 词:缺氧  复合梭曼中毒  PC12细胞  钙离子  CAMP  CAM  CAM-PKⅡ
文章编号:1000-5404(2001)02-0169-03
修稿时间:2000年4月25日

Concentration of Ca2+, contents of cAMP, CaM and Ca2+/CaM-PK II in pheochro-mocytoma PC12 cells after combined soman and hypoxia injury
ZHAO Ji-qing,WU Qiang,WANG Shi-li,WEI Xiang-de,DONG Zhao-jun,LI Yun-peng,LIU Yong.Concentration of Ca2+, contents of cAMP, CaM and Ca2+/CaM-PK II in pheochro-mocytoma PC12 cells after combined soman and hypoxia injury[J].Acta Academiae Medicinae Militaris Tertiae,2001,23(2):169-171.
Authors:ZHAO Ji-qing  WU Qiang  WANG Shi-li  WEI Xiang-de  DONG Zhao-jun  LI Yun-peng  LIU Yong
Abstract:Objective To observe the changes of the concentration of Ca2+, contents of cAMP, CaM and activity of Ca2+/CaM-PK II in pheochromocytoma PC12 cells after combined soman and hypoxia injury. Methods The changes of [Ca2+], and activity of CaM, cAMP and Ca2+/CaM-PK II in PC12 cells were studied after combined soman and hypoxia injury with radioimmunoassay. Results The changes of [Ca2+], the contents of CaM, cAMP were significantly higher in hypoxic and soman intoxicated group than in soman intoxicated group and control group under hypoxia; but the activity of Ca2+/CaM-PK Ⅱ were significantly decreased. Conclusion [Ca2+], CaM, cAMP and Ca2+/CaM-PK Ⅱ exert important role in the damage of PC12 after combined soman and hypoxia injury.
Keywords:hypoxia  soman  Ca~( 2+)  overload  CaM
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