| 嗅鞘细胞与聚乳酸-聚羟基乙酸共聚物材料的细胞相容性研究 |
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| 引用本文： | 矫树生,李兵仓,陈建梅,游华.嗅鞘细胞与聚乳酸-聚羟基乙酸共聚物材料的细胞相容性研究[J].第三军医大学学报,2008,30(5):370-373. |
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| 作者姓名： | 矫树生 李兵仓 陈建梅 游华 |
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| 作者单位： | 1. 第三军医大学大坪医院野战外科研究所第六研究室,创伤、烧伤与复合伤国家重点实验室,重庆,400042
2. 南京军区福州总医院骨科,福州,350001 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 目的 探讨嗅鞘细胞(olfactory ensheathing cells,OECs)与聚乳酸-聚羟基乙酸共聚物poly(DL-lactide-co-gly-colide),PLGA]材料的细胞相容性.方法 将新鲜分离的OECs细胞悬液接种于PLGA膜上,然后用倒置相差显微镜、扫描电镜观察细胞生长及细胞与材料的附着情况;MTT、流式细胞术等方法 测定OECs的细胞增殖和细胞周期变化.结果 PLGA膜上培养OECs的形态特征、增殖能力、细胞周期及倍体水平等与正常培养的OECs相比,均无显著性差异(P>0.05).结论 PLGA生物材料与OECs相容性好,适于构建组织工程化人工神经.
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| 关 键 词： | 嗅鞘细胞 聚乳酸-聚羟基乙酸共聚物 细胞相容性 人工神经 |
| 文章编号： | 1000-5404（2008）05-0370-04 |
| 收稿时间： | 2007-03-22 |
| 修稿时间： | 2007-12-21 |
Cytocompatibility of biomaterial poly (DL-lactide-co-glycolide) with olfactory ensheathing cells |
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| JIAO Shu-sheng,LI Bing-cang,CHEN Jian-mei,YOU Hua.Cytocompatibility of biomaterial poly (DL-lactide-co-glycolide) with olfactory ensheathing cells[J].Acta Academiae Medicinae Militaris Tertiae,2008,30(5):370-373. |
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| Authors: | JIAO Shu-sheng LI Bing-cang CHEN Jian-mei YOU Hua |
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| Institution: | 1State Key Laboratory of Trauma;Burn and Combined Injury;Department of 6;Research Institute of Surgery;Daping Hospital;Third Military Medical University;Chongqing 400042;2Fuzhou General Hospital of Nanjing Command;Fuzhou 350001;China |
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| Abstract: | Objective To investigate the cytocompatibility between poly (DL-lactide-co-glycolide) (PLGA) and olfactory ensheathing cells (OECs) so as to found a basis to prepare an artificial nerve made of PLGA with OECs. Methods Freshly isolated OECs were seeded on PLGA membrane, and then their growth and adhesion to the membrane were observed at different time points by phase contrast inverted microscopy and scanning electron microscopy. The proliferation and the cell cycle of OECs were examined by MTT and flow cytom... |
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