| 利用简并PCR克隆烟草节杆菌02181肌酸酶基因 |
| |
| 引用本文： | 智强,李淑慧,高利宏,李鹏,周玉,易维京,王源,胡川闽.利用简并PCR克隆烟草节杆菌02181肌酸酶基因[J].第三军医大学学报,2007,29(12):1221-1223. |
| |
| 作者姓名： | 智强 李淑慧 高利宏 李鹏 周玉 易维京 王源 胡川闽 |
| |
| 作者单位： | 第三军医大学医学检验系临床生物化学教研室,重庆,400038 |
| |
| 基金项目： | 第三军医大学校科研和教改项目 |
| |
| 摘 要： | 目的 用简并PCR从烟草节杆菌02181中扩境出肌酸酶部分序列,以期获得其全长编码基因.方法 从NCBI查询已发表的肌酸酶蛋白序列,将其递交到Bolck Maker服务器,利用工具软件CODEHOP设计简并引物,以烟草节杆菌02181基因组DNA为模板做PCR.结果 将所得目的片段(414 bp)在NCBI上BLASTx,结果表明,所得序列与不同菌种来源的肌酸酶高度同源,其中与节杆菌属肌酸酶基因的同源性最高,一致性达到了71%.结论 从烟草节杆菌02181中成功克隆出了肌酸酶基因的部分序列.
|
| 关 键 词： | 肌酸酶 简并PCR |
| 文章编号： | 1000-5404（2007）12-1221-03 |
| 修稿时间： | 2007-01-19 |
Gene cloning of creatinase from Arthrobacter Nicotianae 02181 using degenerate PCR |
| |
| ZHI Qiang,LI Shu-hui,GAO Li-hong,LI Peng,ZHOU Yu,YI Wei-jing,WANG Yuan,HU Chuan-min.Gene cloning of creatinase from Arthrobacter Nicotianae 02181 using degenerate PCR[J].Acta Academiae Medicinae Militaris Tertiae,2007,29(12):1221-1223. |
| |
| Authors: | ZHI Qiang LI Shu-hui GAO Li-hong LI Peng ZHOU Yu YI Wei-jing WANG Yuan HU Chuan-min |
| |
| Institution: | Department of Clinical Biochemistry, College of Medical Laboratory, Third Military Medical University, Chongqing 400038, China |
| |
| Abstract: | Objective To clone partial sequence of creatinase of Arthrobacter Nicotianae 02181 using degenerate PCR so as to obtain its whole sequence.Methods We inquired the protein codes of the enzyme in NCBI and submitted them to Block Maker,then designed the degenerate primers of partial of the enzyme by CODEHOP.Degenerate PCR was used to obtain the sequence.Results The sequence(414 bp) we got was submitted to BLASTx of NCBI,which was similar to those creatinases from different bacteria.In all these bacteria,the identity between the obtained sequence and that from Arthrobacter sp.FB24 was 71%,indicating we got the right one.Conclusion We cloned partial sequence of creatinase from Arthobacer nicotianae 02181. |
| |
| Keywords: | Block Maker CODEHOP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
