CTLA4Ig基因转染猪皮异种移植的体外实验研究

目的 探索重组腺病毒载体（Recombinant adenovirus,rAdv）介导CTLA4Ig基因转染猪皮异种移植免疫耐受的分子机制。方法 构建CTLA4Ig重组腺病毒载体，转染猪树突状细胞（Dendritic Cell,DC），以猪表皮细胞匀浆液为刺激原，观察DC递呈猪表皮抗原刺激人T细胞增殖、活化及信号转导的影响；同时，以CTLA4Ig转染小鼠DC细胞，观察该DC膜表面分子CD40、CD80表达。结果 CTLA4Ig转染DC能显著抑制人外周血T淋巴细胞增殖能力（P＜0.01）和T细胞肌醇磷脂信号系统转导活性，IL－2分泌亦受到明显抑制（P＜0.05）；转染CTLA4Ig的小鼠DC膜表面分子CD80表达降低（P＜0.05）。结论 腺病毒介导CTLA4Ig基因转染转皮能诱导人外周血T淋巴细胞免疫耐受。

Experimental study on the mechanism of CTLA4Ig transgenic swine derm xenotransplantation in vitro

Objective To explore the molecular mechanism of xenotransplantation immune tolerance by recombinant adenovirus (rAdv) CTLA4Ig transgenic swine derm. Methods A recombinant CTLA4Ig Adenovirus expression vector was constructed and then transfected into swine dendritic cell (DC). The transfected dendritic cell was stimulated with swine derm antigen and cultured with human T lymphocyte. CD80 and CD40 of DC membrane were detected, then function and signal transduction mechanism (PI PLC pathway) of T lymphocyte was observed. Results T lymphocyte proliferation ability and IL 2 secretion were suppressed significantly by the CTLA4Ig rAdv transfected DC, signal transducing activity was also decreased.Conclusion The CTLA4Ig transgenic dendritic cell mediated by recombinant adenovirus vector could induce the immune tolerance for human peripheral T lymphocytes.