低剂量硫芥诱导大鼠脾脏氧化应激状态的改变

目的探讨低剂量硫芥诱导大鼠脾脏氧化应激状态的改变及其意义.方法皮下注射硫芥,梯度离心法分离大鼠脾线粒体.用紫外分光光度法检测ROS、SOD、MDA及细胞色素含量;荧光分光法检测细胞内GSH含量;HE染色法观察脾脏病理损伤.结果低剂量硫芥作用下,脾脏出现水肿;组织和线粒体内ROS、SOD、MDA、GSH、GSSG含量出现不同程度的改变;线粒体电子传递链成分(细胞色素)含量出现不同程度的丢失.结论氧化应激状态的改变是低剂量硫芥诱导机体免疫器官(脾)损伤的毒作用机制之一.

Changes of oxidative stress in rat spleen induced by low-dose sulfur mustard

Objective To study the changes of oxidative stress in rat spleen induced by low-dose sulfur mustard. Methods A total of 30 Wistar rats were divided into control group and sulfur mustard group. The sulfur mustard group was injected sulfur mustard (0.6 LD_ 50, 1.92 mg/kg) subcutaneously, while the control group with the same volume of saline solution. In all rats, the spleen mitochondria were isolated by density gradient centrifugation. The levels of ROS, SOD, MDA and cytochrome were measured by ultraviolet spectrophotometry. The changes of GSH were detected by fluorescence spectrophotometry. The morphology of spleen was investigated with HE staining. Results Treated with low-dose sulfur mustard, the spleen was of edema, the concentrations of ROS, SOD, MDA, GSH and GSSG changed, and the cytochrome (c, c1, aa3, b) of the mitochondria respiration chain lost. Conclusion The change of oxidative stress is one reason for the damage of rat spleen induced by low-dose sulfur mustard.