首页 | 本学科首页   官方微博 | 高级检索  
检索        

苏拉明对肺癌血管生成的抑制作用
引用本文:王海东,王明荣,杨康,杨军民,曾会昌.苏拉明对肺癌血管生成的抑制作用[J].第三军医大学学报,2001,23(5):539-540.
作者姓名:王海东  王明荣  杨康  杨军民  曾会昌
作者单位:第三军医大学附属西南医院胸心外科,
摘    要:目的 研究苏拉明对肺癌血管生成的抑制作用及其机制。方法 采用细胞培养和免疫组织化学的方法离体研究肺癌A5 49细胞、人血管内皮ECV 30 4细胞VEGF及其受体 (Flt、KDR)的表达及苏拉明的影响。结果 苏拉明对肺癌细胞产生和分泌VEGF无影响 ;ECV 30 4细胞KDR、Flt的蛋白表达在苏拉明浓度为 0 .2 5ng/ml和 2 .5ng/ml时 ,受到显著抑制 ;而苏拉明浓度为 0 .2 5ng/ml和 2 5ng/ml时Flt的表达 ,以及苏拉明浓度为 2 .5ng/ml和 2 5ng/ml时KDR的表达则无明显变化 (与对照组比较 ,P >0 .0 5 ) ;苏拉明对A5 49、ECV 30 4细胞增生无影响。结论 苏拉明抑制肺癌血管生成的作用机制可能是通过抑制血管内皮细胞上的VEGF受体表达 ,减少VEGF与其受体结合所致。

关 键 词:苏拉明  肺癌  血管生成  抑制作用
文章编号:1000-5404(2001)05-0539-02
修稿时间:2000年12月24

Study of the inhibitory effect of suramin on the angiogenesis of lung cancer
WANG Hai-dong,WANG Ming-rong,YANG Kang,YANG Jun-ming,ZENG Hui-chang.Study of the inhibitory effect of suramin on the angiogenesis of lung cancer[J].Acta Academiae Medicinae Militaris Tertiae,2001,23(5):539-540.
Authors:WANG Hai-dong  WANG Ming-rong  YANG Kang  YANG Jun-ming  ZENG Hui-chang
Abstract:Objective To investigate the role of suramin in inhibiting the angiogenesis of lung cancer. Methods The protein expression of VEGF and its receptor fms like tyrosine kinase(Flt), kinase insert domain containing receptor(KDR) in lung cancer cell A549 and ECV 304 was studied with immunohistochemical technique. The anti angiogenesis effect of suramin was evaluated with immunohistochemical technique and cell culture. Results Suramin showed no effect on the production of lung cancer cells and their secretion of VEGF. No possitive expression of Flt, KDR in A549 was found in the suramin interfered group and non interfered group, but VEGF was expressed in both groups. The Flt and KDR expressions in ECV 304 cells were decreased significantly after the treatment of suramin at 0.25 ng/ml and 2.5 ng/ml respectively. There was no effect on proliferetion of A549 and ECV 304 after suramin interference. Conclusion The mechanism of inhibiting angiogenesis of suramin is probably due to reducing the VEGF receptor expression in vascular endothelial cells and inhibiting the binding of VEGF and its receptors.
Keywords:suramin  lung cancer  angiogenesis
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号