硫酸软骨素、硫酸肝素对毛乳头细胞粘附及生长的影响

目的：探讨细胞外基质在毛囊生长发育中的作用，寻持毛乳头细胞（DPC）生长的调节因素。方法：用两步酸消化法分离培养DPC，并通过免疫组化α-平滑肌肌动蛋白（Actin）染色证实。测定硫酸软骨素A，硫酸软骨素C及硫酸肝素对DPC粘附及生长的影响。结果：两步酶消化法分离培养DPC效率高，纯度高，方法简单易行。硫酸软骨素A和硫酸肝素明显促进DPC粘附及生长，而硫酸软骨素C作用不明显。结论：某些细胞外基质可调节DPC的生长，从而影响毛囊的生长发育。

Influence on the adhesion and growth of dermal papilla cells by chondroitin sulfate and heparin sulfate

Objective To investigate the actions of extra cellular medium in growth and differentiation of hair follicle and to look for growth adjusting factors for dermal papilla cells (DPC). Methods Dermal papilla cells were isolated and cultivated with two steps method and the cells were identified by immunohistochemical staining for actin. Influence was examined on the adhesion and growth of dermal papilla cells by chondroitin sulfate A, chondroitin sulfate C and heparin sulfate. Results Two steps method of enzyme digestion for isolating and cultivating dermal papilla cells was an efficient method and large amount of dermal papilla of high purity were harvested with this method. The method is very simple and easy to manege with. Increased adhesion and growth of dermal papilla cells were observed in specimen treated with chondroitin A and heparin sulfate. No significant effects was observed in the cells treated with chondroit in sulfate C. Conclusion Some extra cellular medium can regulate the adhesion and growth of dermal papilla cells and therefore influence the growth and development of hair follicle.