| 舱内腹部爆炸伤大鼠血清及肠道组织MDA含量和SOD、GSH-Px活力变化及意义 |
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| 引用本文： | 聂海,黄显凯,赖西南,王丽丽,康建毅,赖军.舱内腹部爆炸伤大鼠血清及肠道组织MDA含量和SOD、GSH-Px活力变化及意义[J].第三军医大学学报,2008,30(10):910-913. |
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| 作者姓名： | 聂海 黄显凯 赖西南 王丽丽 康建毅 赖军 |
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| 作者单位： | 1. 第三军医大学大坪医院野战外科研究所全军战创伤中心,重庆,400042
2. 第三军医大学大坪医院野战外科研究所第六研究室,创伤、烧伤与复合伤国家重点实验室,重庆,400042 |
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| 基金项目： | 解放军医学科研项目，国家重点实验室基金 |
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| 摘 要： | 目的 探讨舱内爆炸致大鼠腹部损伤后血清及肠道组织MDA含量及SOD、GSH-Px活力的动态变化规律.方法 120只SD大鼠完全随机分为舱内组、舱外组,各60只,采用DDNP纸质点爆源在模拟装甲舱室和舱外开阔地爆炸建立腹部爆炸伤模型;另设立正常对照组(10只).爆炸后0.5 h内、3、8、24、48 h和72 h采集血液和肠道组织标本,检测MDA含量和SOD、GSH-Px活性.结果 大鼠爆炸后血清和肠道组织内MDA水平持续升高,伤后0.5 h内升高显著(P＜0.05),3～8 h上升最快,24 h达峰值;舱内组较舱外组升高显著(P＜0.05).SOD、GSH-Px活性在伤后早期略有升高,继而进行性下降,24 h达最低值;舱内组下降速度较舱外组快(P＜0.05).MDA水平达峰值及SOD、GSH-Px活性达低谷后逐渐恢复,舱内组恢复较慢.血清和肠道组织MDA含量及GSH-Px活力呈正相关(P＜0.01).结论 大鼠舱室爆炸致腹部损伤后氧化应激反应较开阔地爆炸出现早、强度大且持续时间长,氧化应激可能参与了大鼠腹部爆炸伤后肠道的继发性损害.检测血清MDA含量和GSH-Px活性可作为伤后肠道组织过氧化损伤的诊断指标之一.
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| 关 键 词： | 创伤与损伤 冲击波 腹部损伤 氧化应激 大鼠 |
| 文章编号： | 1000-5404(2008)10-0910-04 |
| 修稿时间： | 2007年11月26 |
Effect of abdominal injury in rats subjected to explosion in enclosed space on malondialdehyde content SOD and GSH-Px activity and in serum and intestinal tissues |
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| NIE Hai,HUANG Xian-kai,LAI Xi-nan,WANG Li-li,KANG Jian-yi,LAI Jun.Effect of abdominal injury in rats subjected to explosion in enclosed space on malondialdehyde content SOD and GSH-Px activity and in serum and intestinal tissues[J].Acta Academiae Medicinae Militaris Tertiae,2008,30(10):910-913. |
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| Authors: | NIE Hai HUANG Xian-kai LAI Xi-nan WANG Li-li KANG Jian-yi LAI Jun |
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| Institution: | NIE Hai1,HUANG Xian-kai1,LAI Xi-nan2,WANG Li-li2,KANG Jian-yi2,LAI Jun1(1Trauma Center,2State Key Laboratory of Trauma,Burns , Injury,Department 6,Institute of Surgery Research,Daping Hospital,Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Objective To investigate the dynamic changes of and malondialdehyde content SOD and GSH-Px activity in serum and intestinal tissues in rats with abdominal injury caused by explosion.Methods A total of 120 male rats were divided randomly into enclosure group and free-field group,and subjected to explosion with instantaneous electric detonator.Ten uninjured rats served as normal control.Blood and intestinal tissue samples were collected immediately and at 3,8,24,48,72 h after blast injury,respectively.SOD and... |
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| Keywords: | wounds and injuries high-energy shock waves abdominal injuries oxidative stress rat |
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