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| 电凝法制作大鼠脑卒中模型的改良研究 | |
| 引用本文： | 刘海朋,胡永波,张小东,王娜,谢鹏.电凝法制作大鼠脑卒中模型的改良研究[J].第三军医大学学报,2011,33(17). |
| 作者姓名： | 刘海朋 胡永波 张小东 王娜 谢鹏 |
| 作者单位： | 1. 重庆医科大学附属第一医院神经内科,重庆,400016 2. 川北医学院附属医院神经内科,南充,637000 3. 华中科技大学同济医学院附属协和医院神经内科,武汉,430022 |
| 基金项目： | 国家重点基础研究发展计划(973计划,2009CB918300)~~ |
| 摘 要： | 目的对电凝制作大鼠脑卒中模型方法进行改良及评价。方法将26只成年雄性SD大鼠(体质量200～300 g)分成5组:Tamura手术组、Tamura假手术组、改良手术一组、改良手术二组、改良假手术组。分别使用Tamura法和改良电凝法制作脑卒中模型。术后对大鼠神经功能缺损,脑梗死面积和部位进行评价。结果与Tamura法比较,改良电凝法手术时间更短,术后大鼠没有出现颞下颌关节紊乱;改良手术一组大鼠没有出现明显的神经功能缺损,改良手术二组大鼠肢体瘫痪比较明显;改良手术一组大鼠脑梗死面积比较小,梗死范围局限于大脑皮层,改良手术二组梗死面积较大,梗死部位在基底节测区和大脑皮层。结论①改良电凝法更加简便快速,对设备和操作技术要求更低,对大鼠其他损伤更小。②使用改良电凝法阻断大脑中动脉的不同部位,可以实现不同面积和部位的脑梗死、满足不同的实验条件和目的。 |
| 关 键 词： | 电凝法 动物模型 大脑中动脉闭塞 脑梗死 大鼠 |
Improved electrocoagulation method for establishing rat cerebral apoplexy model |
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| Liu Haipeng,Hu Yongbo,Zhang Xiaodong,Wang Na,Xie Peng.Improved electrocoagulation method for establishing rat cerebral apoplexy model[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(17). | |
| Authors: | Liu Haipeng Hu Yongbo Zhang Xiaodong Wang Na Xie Peng |
| Institution: | Liu Haipeng1,Hu Yongbo1,Zhang Xiaodong2,Wang Na3,Xie Peng1(1Department of Neurology,First Affiliated Hospital,Chongqing Medical University,Chongqing,400016,2Department of Neurology,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan Province,637000,3Department of Neurology,Union Hospital of Tongji Medical College,Huazhong University of Science and Technology,Wuhan,Hubei Province,430022,China) |
| Abstract: | Objective To improve the electrocoagulation method for establishing a rat cerebral apoplexy model,and to evaluate the improved electrocoagulation method.Methods Rat cerebral apoplexy models were established with Tamura method and an improved electrocoagulation method separately.Twenty-six adult male Sprague-Dawley(SD) rats(body weights 200 to 300 g) were divided into five groups: a Tamura operation group,a Tamura sham-operation group(no middle cerebral artery occlusion),a first improved operation group(midd... |
| Keywords: | electrocoagulation animal models middle cerebral artery occlusion cerebral infarction rats |
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