碳青霉烯类耐药鲍曼不动杆菌分子流行病学及耐药机制的研究

目的 分析我院碳青霉烯类耐药的鲍曼不动杆菌分子流行病学及耐药机制.方法 鲍曼不动杆菌源自2008年1-12月我院临床分离株,共51株.采用K-B法(CLSI 2008年标准)筛选对碳青霉烯类耐药的鲍曼不动杆菌临床分离株,采用随机引物多态性DNA(randomly amplified polymorphism DNA,R...

Molecular epidemiology and drug resistant mechanisms of carbapenem-resistant Acinetobacter baumannii

Objective To investigate the molecular epidemiology and drug resistance mechanism of carbapenem-resistant Acinetobacter baumannii(CRAB).Methods Antimicrobial susceptibility was tested on 51 strains of Acinetobacter baumannii isolated in our hospital from January to December 2008 by Kirby-Bauer method.The results were assessed according to the standards recommended by CLSI(2008).The homology of 51 isolates of CRAB was determined by randomly amplified polymorphism DNA(RAPD).The genotype of TEM,SHV,CTX-M,IMP-1,IMP-2,VIM,VIM-2,OXA-23,OXA-24 and class Ⅰ,Ⅱ integrase(Int-1 and Int-2) was detected by polymerase chain reaction(PCR).Results These 51 strains Acinetobacter baumannii were all multi-drug resistant,and were resistant to most antibiotics except amikacin.Among them,50 strains were the same clone.Positive rate of these strains to TEM,SHV,CTX-M,IMP-1,VIM-2,OXA-23 and Int1 was 98.0%,23.5%,19.6%,25.5%,19.6%,72.5%,and 86.3%,respectively.But no genotype of IMP-2,VIM,OXA-24 and Int-2 was detected.Conclusion The stain of CRAB with TEM,OXA-23,IMP-1,VIM-2 and Int-2 genotypes breaks out in our hospital.Its production of OXA-23 carbapenemase and metallo-β-lactamase might be the main mechanisms of carbapenem resistance.