| 人IL-16在大肠杆菌中的融和表达 |
| |
| 引用本文： | 曹廷兵,叶治家,甘立霞,钟小林,巩燕,彭家和.人IL-16在大肠杆菌中的融和表达[J].第三军医大学学报,2002,24(4):428-430. |
| |
| 作者姓名： | 曹廷兵 叶治家 甘立霞 钟小林 巩燕 彭家和 |
| |
| 作者单位： | 第三军医大学基础医学部生物化学与分子生物学教研室,重庆,400038 |
| |
| 基金项目： | 全军“九五”医药卫生基金资助项目 ( 96L0 31)，重庆市攻关项目( 970 4 3) |
| |
| 摘 要： | 目的 将人IL－16 cDNA克隆至原核融合表达载体Pinpoint^TM Xa-3上，并进行表达研究。方法 含IL－16 cDNA的质粒IL－16／PGEM－3ZF（＋）和Pinpoint^TM Xa－3原核融合表达载体经酶切消化、目的DNA片段的分离、重组连接、筛选、序列分析等系列操作，获得重组质粒Pinpoint^TM－IL－16，将质粒Pinpoint^TMXa-IL-16转化至大肠杆菌JM109，用IPTG诱导表达JM109工程菌，Western blotting检测表达产物。结果 IL－16 cDNA成功克隆至融合表达载体Pinpoint^TM Xa-3,Western blotting检测经诱导含有Pinpoint^TM Xa－IL－16质粒的JM109细菌，有IL－16融合蛋白的表达。结论 成功地表达了IL－16融合蛋白。
|
| 关 键 词： | IL－16 克隆 融合表达 大肠杆菌 |
| 文章编号： | 1000-5404(2002)04-0428-03 |
| 修稿时间： | 2001年4月15日 |
Fusion expression of human interleukin-16 in E. coli |
| |
| CAO Ting bing,YE Zhi jia,GAN Li xia,ZHONG Xiao lin,GONG Yan,PENG Jia he.Fusion expression of human interleukin-16 in E. coli[J].Acta Academiae Medicinae Militaris Tertiae,2002,24(4):428-430. |
| |
| Authors: | CAO Ting bing YE Zhi jia GAN Li xia ZHONG Xiao lin GONG Yan PENG Jia he |
| |
| Abstract: | Objective To construct the plasmid Pinpoint TM Xa IL 16 and then observe the expression of fused IL 16 in E.coli . Methods IL 16 cDNA was isolated from plasmid IL 16/PGEM 3Zf(+) and ligated to the expression vector Pinpoint TM Xa 3 after enzyme digestion, target DNA fragment isolation, recombinant ligation, and screening. After the DNA sequence of plasmid Pinpoint TM Xa IL 16 was analyzed, it was transfected to E. coli strain JM109 with the inducement of IPTG. The expression of IL 16 was identified with Western blotting. Results Pinpoint TM Xa IL 16 was constructed successfully and IL 16 fused with the biotin was expressed in E. coli . Conclusion IL 16 is expressed in E. coli , which found a basis for batch production of the fusion protein. |
| |
| Keywords: | interleukin 16 cloning fusion expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
