双葛解酲丸对酒精性脂肪肝大鼠炎症及脂质代谢的影响

目的探讨双葛解酲丸对酒精性脂肪肝模型的治疗效果。方法将65只SD大鼠分为空白对照组(11只)和酒精性脂肪肝(alcoholic fatty liver,AFL)组(54只),后者给予白酒灌胃建立AFL模型。8周后,处死其中5只(空白对照组1只,AFL组4只)病理组织学检查以验证其造模成功,然后将AFL组进一步分为模型对照组、双葛解酲丸高剂量组、双葛解酲丸中剂量、双葛解酲丸低剂量组和海王金樽治疗组。治疗4周末处死所以大鼠,检测血清肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)、痩素(leptin,LEP)、脂联素(adiponectin,ADP)和游离脂肪酸(free fatty acid,FFA)。同时对肝脏进行病理切片组织学检查。结果双葛解酲丸高剂量组大鼠血清IL-6(62.49±34.16)pg/ml]、TNF-α(3.25±0.20)ng/ml]、FFA(572.16±45.37)μmol/L]和LEP(1.89±0.21)ng/ml]明显低于模型对照组与海王金樽治疗组(P<0.01),血清ADP(3.05±0.27)mg/ml]明显高于模型对照组(2.17±0.37)mg/ml]与海王金樽治疗组(2.69±0.32)mg/ml,P<0.01)],双葛解酲丸对大鼠肝脏的脂肪变性及炎症有明显改善。结论双葛解酲丸对酒精性脂肪肝有治疗作用。

Effects of Shuangge Jiecheng Pill on inflammatory factors and lipid metabolism in alcoholic fatty liver rats

Objective To determine the efficiency of Shuangge Jiecheng Pill on the treatment of alcoholic fatty liver in the rat model.Methods A total of 65 SD rats were randomly divided into normal control group(n=11) and model group(n=54).The rat model of alcoholic fatty liver disease was induced by Chinese liquor(56% Hongxing wine) by intragastric administration.One rat from normal control and 4 from the model group were used for model identification.The rest 50 rats of model group were further divided into Haiwang group(HW group,n=10,gastrically treated by Haiwang Jinzun Pill at 1.5 g/(kg·d),twice per day for 4 weeks),and high-,middle-and low-dosed Shuangge Jiecheng Pill groups.At the end of treatment,all rats were killed and their liver was taken for pathological changes.The serum levels of TNF-α,IL-6,leptin(LEP),adiponectin(ADP) and free fatty acid(FFA) in the rats of all groups were measured and compared.Results High-dosed Shuangge Jiecheng Pill resulted in obvious decrease in serum levels of IL-6(62.49±34.16)pg/ml],TNF-α (3.25±0.20)ng/ml],FFA (572.16±45.37)μmol/L] and LEP (1.89±0.21)ng/ml] when compared with those of normal control and HW group(P<0.01),and significant increase in ADP (3.05±0.27)mg/ml] compared with that of normal control(2.17±0.37)mg/ml] and HW group(2.69±0.32)mg/ml,P<0.01].Pathological observation displayed obvious improvement in liver fatty changes and infliammation in Shuangge Jiecheng Pill treatment groups.Conclusion Shuangge Jiecheng Pill exerts curative effect on alcoholic fatty liver disease in rats.