烧伤后腺垂体生长激素细胞亚微结构立体计量测定的实验研究

本实验利用立体计量学方法,对30％Ⅲ°凝固汽油体表烧伤的家兔腺垂体生长激素细胞亚微结构进行了定量分析,并用氧化酶法测定了血糖,结果表明:(1)烧伤后高尔基复合体、线粒体和粗面内质网体积密度呈双相反应;(2)GH细胞未成熟颗粒的成熟速度加快;(3)烧伤后血糖与GH细胞的某些功能活动相关明显;(4)在对照组GH细胞的自分泌对其胞吐有负反馈作用,而在烧伤组则反之;(5)新生线粒体可能来源于高尔基复合体,新生高尔基复合体可能来源于质膜成份的重利用加强。

Stereology Applied to the Quantitative Study of Ultrastructures of GH Cells

The ultrastructural changes in growth hormone (GH) cells of rabbit adeno-hypophysis are determined quantitatively with stereological method after burn injury. Blood sugar levels are concurrently measured. The results show: the volume fraction of Golgi complex, mitochondria and rough endoplasmic reticulum shows a 2-phase curve after burn, and it may be divided into 3-periods stress, modulation and functional activation and recovery; the rate of maturation of secretory granules is increased; a close relation between the blood sugar and some activities of GH cell secretion after burn presents; the autocrine shows a negative feedback to the exocytosis in the control group,and a positive feedback in burned ones; the newly formed mitochondria may be originated from the new Golgi complex from the reutilization of plasma components.