大鼠颌下腺血管内皮生长因子cDNA的转移及表达

目的：探讨以颌下腺为靶器官,进行血管内皮生长因子（ＶＥＧＦ）基因治疗后,颌下腺中ＶＥＧＦ含量变化。方法：构建了ＶＥＧＦ基因表达的真核细胞表达载体,以阳离子脂质体为介质,向大鼠颌下腺转染外源性的ＶＥＧＦ基因,应用ＲＴ－ＰＣＲ技术观察ＶＥＧＦ基因的表达,ＥＬＩＳＡ方法检测颌下腺中ＶＥＧＦ的含量变化。结果：转ｐＢＫＣＭＶ－ＶＥＧＦ１２１基因后第１天,大鼠颌下腺内ＶＥＧＦ的表达及涎液中ＶＥＧＦ的含量即开始

Expression of vascular endothelial growth factor transferred into salivary glands in rats

Objective: To study the feasibility of using vascular endothelial growth factor (VEGF) gene therapy for salivary glands and observe the changes in content of VEGF in spittle. Methods: VEGF cDNA was cloned into eukaryotic expression vector pBKCMV and the VEGF gene was transferred into rat maxillary glands mediated by lipotect AMINE. The expression of VEGF gene was investigated by RT PCR and the content of VEGF in spittle was observed by ELISA. Results: The expression of VEGF gene and the content of VEGF were significantly higher in the experimental group than in the control. It reached its peak from the 2nd to 3rd d after treatment and remained high for 5 d. Conclusion: After being mediated by lipotect AMINE, the VEGF gene can express steadily and constantly. The content of VEGF in spittle becomes higher. It provides a new way for promoting oral wound healing in clinical practice.