| 组蛋白去乙酰化酶11在诱导大鼠肝移植免疫耐受中的作用 |
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| 引用本文： | 赖星,连峥嵘,陈勇,李金政,廖汪洋,张玉君,龚建平,刘作金.组蛋白去乙酰化酶11在诱导大鼠肝移植免疫耐受中的作用[J].第三军医大学学报,2011,33(4). |
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| 作者姓名： | 赖星 连峥嵘 陈勇 李金政 廖汪洋 张玉君 龚建平 刘作金 |
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| 作者单位： | 重庆医科大学附属第二医院肝胆外科;重庆医科大学附属第二医院肝胆外科400010重庆;第三军医大学西南医院全军肝胆外科研究所; |
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| 基金项目： | 国家自然科学基金(30972888,30801126)~~ |
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| 摘 要： | 目的通过观察组蛋白去乙酰化酶11(histone deacetylase 11,HDAC11)及IL-10表达水平在3种大鼠肝移植模型中的变化,探讨肝移植免疫耐受形成的相关机制。方法纯系Lewis和BN大鼠各30只,建立肝移植排斥模型后将受体分为排斥组(Lewis-BN)、免疫抑制剂干预组(Lewis-BN)、耐受组(BN-lewis),每组10只。干预组于术后1~7 d以1 mg/kg肌肉注射他克莫司(FK506);分别于术后7 d处死受体。移植物排斥反应程度采用Banff评分标准;免疫荧光组织化学观察HDAC11表达情况;实时荧光定量PCR及Western blot测定肝组织HDAC11 mRNA和蛋白表达水平;酶联免疫吸附法检测血清IL-10及IFN-γ的含量。结果术后7 d,排斥组为严重的急性排斥反应,Banff评分Ⅲ级,干预组为Ⅱ级,耐受组为0~I级。排斥组HDAC11 mRNA及蛋白表达的水平明显高于干预组和耐受组(P<0.05),耐受组表达量最低,与干预组有统计学差异(P<0.05)。排斥组IL-10表达明显低于干预组和耐受组(P<0.05);而IFN-γ含量排斥组明显高于干预组和耐受组(P<0...
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| 关 键 词： | 组蛋白去乙酰化酶11 肝移植 免疫耐受 大鼠 |
Role of HDAC11 in induction of immune tolerance in rats after liver transplantation |
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| Lai Xing,Lian Zhengrong,Chen Yong,Li Jinzheng,Liao Wangyang,Zhang Yujun,Gong Jianping,Liu Zuojin.Role of HDAC11 in induction of immune tolerance in rats after liver transplantation[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(4). |
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| Authors: | Lai Xing Lian Zhengrong Chen Yong Li Jinzheng Liao Wangyang Zhang Yujun Gong Jianping Liu Zuojin |
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| Institution: | Lai Xing1,Lian Zhengrong1,Chen Yong1,Li Jinzheng1,Liao Wangyang1,Zhang Yujun2,Gong Jianping1,Liu Zuojin1(1Department of Hepatobiliary Surgery,Second Affiliated Hospital,Chongqing Medical University,Chongqing,400010,2Institute of Hepatobiliary Surgery,Southwest Hospital,Third Military Medical University,400038,China) |
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| Abstract: | Objective To observe the changes of histone deacetylase 11(HDAC11) and IL-10 in the 3 rat liver transplantation models,and to explore the related mechanism of the formation of immune tolerance.Methods Thirty pure Lewis and thirty BN rats were used to establish liver transplantation,including 20 pairs of Lewis to BN and 10 pairs of BN to lewis.The recipients were divided into 3 groups: rejection group(Lewis to BN,n=10),immunosuppressant intervention group(Lewis to BN,n=10) and tolerance group(BN to Lewis,n=1... |
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| Keywords: | histone deacetylase 11 liver transplantation immune tolerance rats |
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