| 正常人脾脏树突状细胞的分布及形态学观察 |
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| 引用本文： | 刘茜,陆江阳,王晓虹,杨毅,李玲.正常人脾脏树突状细胞的分布及形态学观察[J].第三军医大学学报,2009,31(9). |
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| 作者姓名： | 刘茜 陆江阳 王晓虹 杨毅 李玲 |
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| 作者单位： | 1. 军事医学科学院放射与辐射医学研究所,北京,100850;解放军总医院第一附属医院病理科,北京,100037
2. 解放军总医院第一附属医院病理科,北京,100037 |
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| 基金项目： | 全军医学科研计划面上项目 |
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| 摘 要： | 目的 认识不同表面分子标记物所标记的脾脏树突状细胞(dendritic cell,DC)分布、成熟程度与超微结构特点.方法 运用光镜、电镜观察与免疫组化标记(CD11c、CD205、CD209、S-100、CD1a、HLA-DR、CDS0及CD86)方法观察35例正常人脾脏组织中的树突状细胞的形态分布及超微结构特点.结果 正常人脾脏树突状细胞主要分布在白髓边缘区和T细胞区,CD11c、CD205和CD209是目前较特异的树突状细胞标志物,标记阳性率分别为4.19%、3.96%和3.02%,其中CD209+DC分布更靠近红髓.树突状细胞表达共刺激分子CD80与CD86的阳性率分别为2.68%和2.02%,主要分布在T细胞区.树突状细胞超微结构特点为细胞体积较大,呈细长状或不规则形,表面可见数个突起向周围细胞间隙延伸;胞核形状不规则,表面有凹陷,可见核仁;胞浆致密或呈低电子密度,细胞器欠发达.结论 正常人脾脏中不同表面分子阳性树突状细胞的比率和分布有所不同,其中大部分为不成熟的树突状细胞,具有活性的成熟树突状细胞数目较少.
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| 关 键 词： | 树突状细胞 脾脏 共刺激分子 人 |
Distribution and morphology of human splenic dendritic cells |
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| LIU Qian,LU Jiang-yang,WANG Xiao-hong,YANG Yi,LI Ling.Distribution and morphology of human splenic dendritic cells[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(9). |
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| Authors: | LIU Qian LU Jiang-yang WANG Xiao-hong YANG Yi LI Ling |
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| Institution: | LIU Qian1,2,LU Jiang-yang2,WANG Xiao-hong2,YANG Yi2,LI Ling2 (1Institute of Radiation Medicine,Academy of Military Medical Sciences,Beijing 100850,2Department of Pathology,First Hospital Affiliated to the General Hospital of PLA,Beijing 100037,China) |
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| Abstract: | Objective To observe the distribution of human splenic dendritic cells (DCs) with several DC markers and ultramicrostructural features of these DCs. MethodsSpleen sample tissues of 35 individuals, including 30 patients after acute traumatic splenic rupture and 5 corpses after sudden death due to cardiac or cerebrovascular diseases were collected. The splenic dendritic cells in these sample sections were observed and analyzed by light microscopy, electron microscopy, and immunohistochemical staining with DCs... |
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| Keywords: | dendritic cells spleen co-stimulatory molecules human |
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