| 青蒿琥酯对人表皮鳞癌A431细胞增殖和凋亡的影响 |
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| 引用本文： | 江忠勇,梅峰,于彬,韩鹃,田衍平,李世峰,周德山.青蒿琥酯对人表皮鳞癌A431细胞增殖和凋亡的影响[J].第三军医大学学报,2009,31(7). |
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| 作者姓名： | 江忠勇 梅峰 于彬 韩鹃 田衍平 李世峰 周德山 |
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| 作者单位： | 第三军医大学基础医学部组织胚胎学教研室,重庆市神经科学研究所,重庆,400038 |
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| 摘 要： | 目的 初步探讨青蒿琥酯(artesunate,ART)对人表皮鳞癌细胞增殖和凋亡的影响及其相关机制.方法 以人表皮鳞癌细胞系A431为研究对象,人永生化角质形成细胞系HaCaT为正常对照,顺铂(diamminedichloroplatinum,DDP)为药物阳性对照,通过MTT法和流式细胞术观察ART对A431细胞增殖和凋亡的影响,以及铁离子抑制剂去铁敏(desferrioxamine.DFOM)部分阻断ART对A431细胞的生长抑制作用.结果 ART对A431细胞有较明显的增殖抑制作用,对HaCaT细胞的损害显著低于DDP;药物组ART60 μmol/L(IC50)作用A431细胞48 h,A431细胞阻滞于S期(67.60±4.12)%,P<0.05],同时凋亡率上调(19.87±0.03)%,P<0.01];药物阻断组DFOM 60μmol/L预处理A431细胞4 h、加入ART60 μmol/L作用钙h,凋亡率显著下调(7.37±0.02)%,P<0.01],而周期检测未发生变化.结论 ART通过阻滞A431细胞停滞于S期和诱导A431细胞凋亡发挥增殖抑制作用,此作用在一定范围内与药物剂量呈正相关;ART对A431细胞的诱导凋亡作用与Fe2+介导有关;ART毒副作用明显低于DDP.
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| 关 键 词： | 青蒿琥酯 鳞状细胞癌 角质形成细胞 细胞凋亡 |
Artesunate inhibits proliferation and apoptosis of epidermal squamous cell line A431 |
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| JIANG Zhong-yong,MEI Feng,YU Bin,HAN Juan,TIAN Yan-ping,LI Shi-feng,ZHOU De-shan.Artesunate inhibits proliferation and apoptosis of epidermal squamous cell line A431[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(7). |
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| Authors: | JIANG Zhong-yong MEI Feng YU Bin HAN Juan TIAN Yan-ping LI Shi-feng ZHOU De-shan |
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| Institution: | JIANG Zhong-yong,MEI Feng,YU Bin,HAN Juan,TIAN Yan-ping,LI Shi-feng,ZHOU De-shan(Department of Histo-logy and Embryology,College of Basic Medical Sciences,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To investigate the effects and the underlying mechanisms of artesunate(ART)on the growth and apoptosis of epidermal carcinoma cells.Methods The proliferation,cell cycle and apoptosis of A431 cells(a cell line of malignant epidermal carcinoma)were detected with MTT assay and flow cytometer(FCM).HaCaT cells(an immortalized keratinocyte cell line)served as normal control,and diamminedichloroplatinum(DDP)as drug positive control.Deferoxamine mesylate(DFOM)was employed to block the effect of ART.Result... |
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| Keywords: | artesunate squamous cell carcinoma keratinocyte cells apoptosis |
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