| 骨髓间充质干细胞与胶质瘤细胞非接触共培养后相关生物学特性分析 |
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| 引用本文： | 张亚兰,朱静,田杰,刘官信,张晓萍,邓兵.骨髓间充质干细胞与胶质瘤细胞非接触共培养后相关生物学特性分析[J].第三军医大学学报,2010,32(7). |
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| 作者姓名： | 张亚兰 朱静 田杰 刘官信 张晓萍 邓兵 |
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| 作者单位： | 1. 重庆医科大学附属儿童医院,儿科研究所,重庆市重点实验室,重庆,400014
2. 重庆医科大学附属儿童医院,心血管内科,重庆,400014 |
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| 基金项目： | 国家自然科学基金(30670871)~~ |
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| 摘 要： | 目的在体外通过大鼠胶质瘤C6细胞与大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)非接触共培养,以探明BMSCs是否受到肿瘤微环境的作用获得肿瘤细胞的相关生物学特性。方法通过6孔板结合Transwell小室建立BMSCs和C6胶质瘤细胞的共培养体系,以共培养组为实验组,设单独培养的BMSCs为对照组;相差显微镜下观察培养后2组细胞形态学的改变;核干细胞因子(nucleostemin,NS)检测细胞增殖力的变化;荧光定量PCR和Western blot检测细胞中mdm2、p53mRNA水平及其蛋白的变化;免疫荧光法检测神经胶质细胞特异的胶质纤维酸蛋白(glial fibrillary acidic protein,GFAP)在细胞中的定位及表达。结果共培养后实验组细胞呈现类似胶质瘤细胞样的形态,表达神经胶质细胞特异的GFAP蛋白;NS蛋白反映的细胞增殖能力未发生改变;共培养后实验组p53mRNA水平明显低于对照组(P<0.01),而p53蛋白的表达水平显著高于对照组(1.63±0.31)vs(0.85±0.12),P<0.05];实验组mdm2mRNA及...
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| 关 键 词： | 骨髓间充质干细胞 胶质瘤细胞 非接触共培养 GFAP蛋白 |
Biological characteristics of rat mesenchymal stem cells non-contact co-cultured with glioma cells |
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| Zhang Yalan,Zhu Jing,Tian Jie,Liu Guanxin,Zhang Xiaoping,Deng Bing.Biological characteristics of rat mesenchymal stem cells non-contact co-cultured with glioma cells[J].Acta Academiae Medicinae Militaris Tertiae,2010,32(7). |
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| Authors: | Zhang Yalan Zhu Jing Tian Jie Liu Guanxin Zhang Xiaoping Deng Bing |
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| Institution: | 1Pediatric Research Institute;2Department of Cardiovasology;Children's Hospital;Chongqing Medical University;Chongqing;400014;China |
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| Abstract: | Objective To study whether rat bone marrow-derived mesenchymal stem cells(BMSCs)obtain tumor characteristics when co-cultured with rat glioma cells by non-contact culture in vitro.MethodsA co-cultural system was established between rat BMSCs and rat glioma cell line C6 by using 6-well plate and Transwell chamber.Cells were divided into 2 groups,experimental group(co-cultural BMSCs),and control group(BMSCs cultured alone).The morphological changes of BMSCs were observed by phase-contrast microscopy.Cell prol... |
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| Keywords: | marrow-derived mesenchymal stem cells rat glioma cells non-contact co-culture GFAP protein |
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