| 2007-2008年北京地区CA16VP1区系统进化分析 |
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| 引用本文： | 王娟,罗珍,范东瀛,高娜,朱俊萍,安静.2007-2008年北京地区CA16VP1区系统进化分析[J].第三军医大学学报,2009,31(23). |
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| 作者姓名： | 王娟 罗珍 范东瀛 高娜 朱俊萍 安静 |
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| 作者单位： | 王娟,范东瀛,朱俊萍,安静,WANG Juan,FAN Dong-ying,ZHU Jun-ping,AN Jing(基础医学院微生物学教研室,北京,100069);罗珍,LUO Zhen(附属北京儿童医院皮肤科,北京,10005);高娜,GAO Na(第三军医大学基础医学部组织胚胎学教研室,重庆市神经科学研究所,重庆,400038) |
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| 基金项目： | 北京市自然科学基金，北京市属高等学校人才强教计划 |
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| 摘 要： | 目的 了解2007-2008年北京地区柯萨奇病毒A组16型(Coxsaekievirus A16,CA16)流行情况及其种系进化规律和基因分型.方法 收集北京地区2007-2008年133例手足13病病例临床标本,从中筛选CAl6毒株,扩增部分毒株VP1区并进行序列测定和分析.结果 共筛选得到39例45份CA16阳性标本,测定其中16株病毒的VP1区核苷酸序列.其同源性为91.43%～98.65%,氨基酸ILd源性为97.98%～100%.该16株病毒与GenBank中选取的24株CA16参考株的核苷酸同源性为78.13%～98.65%,氨基酸同源性为89.9%～100%.16株北京分离株中的9株与近年多数中国大陆分离株形成进化树上亲缘关系较近的一群,其余7株与我国台湾及周边国家的一系列分离株亲缘关系较近.结论 CA16为北京地区手足口病的主要致病原之一,本地区毒株同源性较高,同GenBank中24株CA16参比毒株序列进行系统进化分析显示其属于B基因群,16株北京分离株在系统进化树七有分别归属于两支的趋势.
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| 关 键 词： | 柯萨奇病毒A组16型 手足口病 系统进化分析 |
Phylogenetic analysis based on VP1 gene of coxsackievirus A16 strains isolated in Beijing from 2007 to 2008 |
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| WANG Juan,LUO Zhen,FAN Dong-ying,GAO Na,ZHU Jun-ping,AN Jing.Phylogenetic analysis based on VP1 gene of coxsackievirus A16 strains isolated in Beijing from 2007 to 2008[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(23). |
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| Authors: | WANG Juan LUO Zhen FAN Dong-ying GAO Na ZHU Jun-ping AN Jing |
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| Institution: | WANG Juan1,LUO Zhen2,FAN Dong-ying1,GAO Na3,ZHU Jun-ping1,AN Jing1 (1Department of Microbiology,School of Basic Medical Sciences,Capital Medical University,Beijing 100069,2Department of Dermatology,Beijing Children's Hospital,Beijing 100045,3Department of Microbiology,College of Basic Medical Sciences,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To investigate the epidemic situation and the phylogenetic relationship of Coxsackievirus A16 (CA16) in Beijing from 2007 to 2008. Methods CA16 strains were isolated from the clinical specimens of 133 patients with hand-foot-mouth disease (HFMD). VP1 gene fragments were amplified by RT-PCR. The produced fragments were sequenced and analyzed. Results Totally 45 CA16 positive specimens were identified from 39 patients and the entire VP1 genes of 16 CA16 strains were sequenced. For these 16 strains,t... |
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| Keywords: | Coxsackievirus A16 hand-foot-mouth disease phylogenetic analysis |
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