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10个品系小鼠的AFLP分析
引用本文:梁智勇,史景泉,魏泓,吴丰春.10个品系小鼠的AFLP分析[J].第三军医大学学报,2003,25(2):155-159.
作者姓名:梁智勇  史景泉  魏泓  吴丰春
作者单位:1. 第三军医大学,附属西南医院病理学研究所,重庆,400038
2. 第三军医大学,实验动物中心,重庆,400038
基金项目:国家自然科学基金资助项目 ( 396 0 0 0 79,3990 0 173),全军“九五”医药卫生科研基金资助项目 ( 96M0 83),重庆市攻关项目
摘    要:目的:实验用小鼠的遗传质量分析和品系鉴定。方法:应用AFLP技术对10个品系小鼠进行分析。结果:共应用25条单酶切引物、25对双酶切引物进行检测,其中17条单酶切AFLP引物、20对双酶切AFLP引物检测到10个品系小鼠的多态性变化。单酶切AFLP共扩出251条带,片段大小在100-2000bp,共得到89个多态位点,占总扩增带的35.5%。双酶切AFLP在50-600bp扩增出清晰条带,统计1507条带,共得到378个多态位点,占总扩增带的25.1%。对多态性片段进行统计,计算出不同品系间的相似指数和遗传距离指数。结果表明昆明鼠(KM)与TA2、BALB/c、BALB/c-nu关系较近;所有品系间关系最近的两品系为BALB/c和BALB/c-nu;关系较远的是DBA/2与T739、615、C57BL/6J,与小鼠品系来源及培育史相符。结论:通过筛选获得特异性AFLP标记,可用以区分遗传背景或外观形态很相似的品系。为小鼠的遗传质量分析和品系鉴定提供了参考资料。

关 键 词:小鼠  遗传多态性  AFLP  实验动物  品系鉴定
文章编号:1000-5404(2003)02-0155-05
修稿时间:2002年2月21日

AFLP analysis of 10 strains of mice
LIANG Zhi yong,SHI Jing quan,WEI Hong,WU Feng chun.AFLP analysis of 10 strains of mice[J].Acta Academiae Medicinae Militaris Tertiae,2003,25(2):155-159.
Authors:LIANG Zhi yong  SHI Jing quan  WEI Hong  WU Feng chun
Abstract:Objective To analyze the genetic quality and to determine the strain of laboratory mice. Methods Ten strains of mice were analyzed by using AFLP method. Results Polymorphism was detected in 10 strains of mice by 17 single enzyme primers and 20 pairs of double enzyme primers amplification. A total of 251 bands were shown by single enzyme AFLP in agarose gel with the size of the bands ranging from 100 bp to 2 000 bp and 89 polymorphic loci were detected. A total of 1507 clear bands between 50 bp and 600 bp were shown by double enzyme AFLP and 378 polymorphic loci were detected. Through statistical analysis, we calculated the similar index and genetic distance index. Our results showed BALB/c and BALB/c nu had the closest relationship and KM had a closer relationship with TA2, BALB/c and BALB/c nu, while DBA/2 showed a distant relationship with T739, 615 and C57BL/6J, coinciding with the origins of breeds. Conclusion Each strain could be distinguished from others by using the AFLP polymorphic primers, which provides reference data for genetic quality analysis and strain determination of mice.
Keywords:mice  genetic polymorphism  AFLP
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