新型冠状病毒S蛋白B细胞表位的预测与分析

目的：分析新型冠状病毒（SARS-CoV-2）S刺突蛋白的二级结构并预测其可能的B细胞表位。方法：基于SARS-CoV-2 S蛋白氨基酸序列，分别采用SOPMA、GOR方法预测S蛋白的二级结构，并结合其跨膜区、亲水性、表面可及性和抗原性参数等综合分析，预测SARS-CoV-2 S蛋白可能的B细胞表位。结果：对SARS-CoV-2 S蛋白二级结构的预测表明，其二级结构中柔性区域以无规卷曲为主，少见转角。多种参数综合分析推测，其可能的优势B细胞表位位于354～360、437～445、454～471、527～537、567～582、678～685、772～779和806～818位氨基酸。结论：采用多参数预测SARS-CoV-2 S蛋白的B细胞表位，为深入研究S蛋白的功能奠定基础。

Prediction and analysis of B-cell epitopes of SARS-CoV-2 S protein

Objective: To predict the secondary structure and B-cell epitopes of S protein of SARS-CoV-2. Methods: The amino acids of SARS-CoV-2 S protein were selected, and the secondary structure was predicted by SOPMA and GOR methods. A combined comprehensive analysis of transmembrane domain, hydrophilicity profile, surface probability and antigenicity index was further made to predict B-cell epitopes of S protein. Results: Prediction of the secondary structure of SARS-CoV-2 S protein indicated that random coils were the main structural type of the flexible region in secondary structure. The predominant B-cell epitopes were probably in the regions of 354-360, 437-445, 454-471, 527-537, 567-582, 678-685, 772-779 and 806-818 amino acids. Conclusion: The B-cell epitope of SARS-CoV-2 S protein can be predicted by multi parameters, which lays foundations for further study of the function of S protein.