兔VX2肝癌模型改良制作及TAE前后CT与DSA影像学评估

目的进一步完善兔VX2肝癌模型的制作方法,并应用CT及DSA检查对肿瘤TAE前后进行影像学评估。方法制作兔VX2肝癌模型20只并保种传代,于接种后第7、14日行CT平扫+增强扫描;第15日行开腹门静脉直接穿刺造影及经导管股-肝动脉造影检查,肿瘤供血动脉行碘油栓塞;第21日再次行CT平扫+增强扫描;第22天开腹行门静脉及肝动脉直接穿刺DSA检查。结果种植2周时肝区肿瘤可全部清楚的展示,CT平扫可见肝区结节状低密度病灶,增强扫描肿瘤在动脉期可呈明显环形强化,门脉期呈低密度。3周时肿瘤坏死明显。DSA显示肿瘤富血供。结论兔VX2肝癌模型改良制作成功率较高。TAE前后CT与DSA表现一致,且肿瘤的血供主要来自肝动脉,门静脉不参与供血。

Improvement of Rabbit VX2 Liver Cancer Model and Imaging Evaluation of CT and DSA Before and After TAE

Objective To improve the production methods of rabbit VX2 liver cancer model, and applications of DSA and CT before and after tumor TAE radiologic evaluation.Methods Production and storage of rabbit VX2 liver cancer model 20 batches. In7,14 days after inoculation CT scan and enhanced scan. 15th of line open portal vein puncture angiography and directly after catheter-hepatic artery angiography examination, the tumor blood supply artery iodine oil embolization. 21 days CT scan and enhanced scanning again. 22 days open lines of portal vein and hepatic artery puncture DSA examination directly.Results When planting 2 weeks liver area tumor can all show clearly, CT scan shows nodular liver area low density lesions, enhanced scanning tumor in can show clear ring arterial enhancement,portal venous phase in low density. 3 weeks when tumor necrosis is obvious. DSA shows the rich blood supply of the tumor.Conclusion Improved the rabbit VX2 liver cancer model success rate is higher. CT and DSA was consistent before and after TAE, and the flow of blood to the tumor mainly comes from the hepatic artery, the portal vein is not involved in blood supply.