外源性Ⅰ型胶原对人胚骨膜成骨细胞生物学特性的影响 |
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摘 要: |
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关 键 词: | 成骨细胞 细胞外基质 Ⅰ型胶原 组织工程 生物学特性 人工骨 |
The influence of type I collagen on the cell behavior of human embryonic periosteous osteoblasts] |
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Authors: | Z Yang X Yu F Huang H Xie |
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Institution: | Department of Orthopedic Surgery, First Affiliated Hospital, WCUMS, Chengdu 610041, China. |
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Abstract: | OBJECTIVE: To study the influence of type I collagen (COL I) on the cell behavior of human periosteous osteoblasts (OB) and the application of type I collagen in constructing bioactive artifical bone. METHODS: OB were cultured on dishes coated with bovine type I collagen in different final concentrations. The cell adhesion was examined by the methods of cell count, the proliferation of OB was studied by 3H-TdR, and the osteoblastic ability was assessed by the synthesis of collagen, osteocalcin and alkaline phosphatase (ALP). RESULTS: OB cultured on type I collagen layer had the following characteristics: 1. The amounts of adhesive cells were maximal top in 25 micrograms/ml final concentration; 2. The proliferation of OB was decreased above 12.5 micrograms/ml final concentration (P < 0.05); 3. The synthesis of type I collagen was reduced slightly (above 25 micrograms/ml, P < 0.05); 4. The secretion of osteocalcin was increased markedly (above 6.25 micrograms/ml, P < 0.05, which reached maximally in 25 micrograms/ml); 5. The ALP activity was also increased (above 12.5 micrograms/ml, P < 0.05). CONCLUSION: Type I collagen promotes the expression of osteoblastic phenotype and cell adhesion. When the scaffold materials for bone tissue engineering are coated with type I collagen, the osteogenesis of OB is enhanced to accelerate the transformation course from artificial bone to biological bone, the best final concentration is 25 micrograms/ml. |
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