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胃泌素,生长抑素对人胃癌细胞生长的调节作用
引用本文:刘小菁,张正.胃泌素,生长抑素对人胃癌细胞生长的调节作用[J].华西医科大学学报,1998,29(3):292-294.
作者姓名:刘小菁  张正
摘    要:采用MTT比色法检测细胞增殖情况,用放射法(RIA)测定胰岛素、胰高血糖素和胃泌素含量,氨基己糖含量用Neuhaus法测定,以期观察五肽胃泌素和生长抑素对体外培养的人胃癌细胞HGC803和HGC823生长的调节作用。结果显示,胃泌素对两株胃癌细胞增殖均有促进作用,以1×10^-5mol/L浓度最为明显,并使胃癌细胞氨基己糖含量明显增加(7.58±0.66vs4.2±0.39pg/细胞,P〈0.05

关 键 词:癌细胞  生长抑素  胃肿瘤  胃泌素  细胞增殖

Regulative effects pentagastrin and somatostatin on growth of human gastric cancer cells in vitro]
X Liu,Z Zhang,L Yang,O Qiang.Regulative effects pentagastrin and somatostatin on growth of human gastric cancer cells in vitro][J].Journal of West China University of Medical Sciences,1998,29(3):292-294.
Authors:X Liu  Z Zhang  L Yang  O Qiang
Institution:Internal Medicine Department, First Affiliated Hospital, Chengdu.
Abstract:In order to observe the regulative effects of pentagastrin (PG) and somatostatin (SS) on the growth of two human gastric cancer cell lines (HGC803 and HGC823) in vitro, we observed the effects of PG and SS on proliferation of human gastric cancer cells by means of MTT. The contents such as gastrin, insulin, and glucagon were determined by radioimmunoassay (RIA), and the hexosamine content was determined by Neuhaus' method. The results showed that the growth of the two human gastric cancer cell lines were obviously promoted by PG. On the contrary, the growth and secretion of gastrin and glucagon were inhibited by SS. In addition, the hexosamine content of human gastric cancer cells was significantly increased by PG (7.58 +/- 0.66 versus 4.20 +/- 0.39 pg/cell, (P < 0.05). But the hexosamine content was decreased by SS (2.62 +/- 0.29 versus 4.20 +/- 0.39 pg/cell, P < 0.05). These findings indicate that the growth of gastric cancer cells is regulated by PG and SS, nevertheless a host of problems need to be elucidated.
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